Three-Dimensional-Construct Bioreactor Conditioning in Human Tendon Tissue Engineering

Human tendon tissue engineering attempts to address the shortage of autologous tendon material arising from mutilating injuries and diseases of the hand and forearm. It is important to maximize the tissue-engineered construct's (TEC's) biomechanical properties to ensure that the construct...

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Bibliographic Details
Published in:Tissue engineering. Part A 2011-10, Vol.17 (19-20), p.2561-2572
Main Authors: Woon, Colin Y.L., Kraus, Armin, Raghavan, Shyam S., Pridgen, Brian C., Megerle, Kai, Pham, Hung, Chang, James
Format: Article
Language:English
Subjects:
Biomechanical Phenomena - physiology
Biomechanics
Biomedical engineering
Bioreactors
Elastic Modulus - physiology
Humans
Original Articles
Physiological aspects
Tendons
Tendons - physiology
Tensile Strength - physiology
Tissue engineering
Tissue Engineering - instrumentation
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Human tendon tissue engineering attempts to address the shortage of autologous tendon material arising from mutilating injuries and diseases of the hand and forearm. It is important to maximize the tissue-engineered construct's (TEC's) biomechanical properties to ensure that the construct is in its strongest possible state before reimplantation. In this study, we sought to determine the bioreactor treatment parameters that affect these properties. Using small- and large-chamber three-dimensional-construct bioreactors (SCB and LCB, respectively), we applied cyclic axial load to TECs comprising reseeded human flexor and extensor tendons of the hand. First, small-sample pilot studies using the LCB were performed on matched-paired full-length flexor tendons to establish proof of concept. Next, large-sample studies using the SCB were performed on matched-paired extensor tendon segments to determine how reseeding, load duty cycle, load magnitude, conditioning duration, and testing delay affected ultimate tensile stress (UTS) and elastic modulus (EM). We found that compared with reseeded matched-paired controls under dynamic-loading at 1.25 N per TEC for 5 days, (1) acellular TECs had lower UTS ( p =0.04) and EM ( p
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2010.0701