Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2011-11, Vol.119 (11), p.741-749
• Main Authors: HOLTEN-ANDERSEN, LARS, THAYSEN-ANDERSEN, MORTEN, JENSEN, SIRI BEIER, BUCHWALD, CHRISTIAN, HØJRUP, PETER, OFFENBERG, HANNE, NIELSEN, HANS JØRGEN, BRÜNNER, NILS, NAUNTOFTE, BIRGITTE, REIBEL, JESPER
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Glycosylation; glycosylation profile; Humans; Immunohistochemistry; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Oral pathology; protein expression; RNA, Messenger - analysis; Saliva - chemistry; TIMP-1; Tissue Inhibitor of Metalloproteinase-1 - analysis; Tissue Inhibitor of Metalloproteinase-1 - genetics; Tissue Inhibitor of Metalloproteinase-1 - metabolism
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1111/j.1600-0463.2011.02796.x

Holten‐Andersen L, Thaysen‐Andersen M, Jensen SB, Buchwald C, Højrup P, Offenberg H, Nielsen HJ, Brünner N, Nauntofte B, Reibel J. Salivary tissue inhibitor of metalloproteinases‐1 localization and glycosylation profile analysis. APMIS 2011; 119: 741–9. Salivary tissue inhibitor of metalloproteinases‐1 (TIMP‐1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP‐1 in saliva remains unknown. Hence, we studied the localization of TIMP‐1 in salivary gland tissue and also investigated if TIMP‐1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP‐1 mRNA and protein expression. To assess TIMP‐1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI‐TOF mass spectrometry. TIMP‐1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP‐1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP‐1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP‐1 and plasma TIMP‐1. Although no clear evidence of TIMP‐1 transcription in major salivary glands was demonstrated our results suggest that TIMP‐1 in saliva does not originate from plasma.