Notch-1 and Notch-4 Receptors as Prognostic Markers in Breast Cancer

Background: Studies looking at immunohistochemical (IHC) staining of Notch receptors in breast cancer and correlation with known prognostic factors are sparse. Methods: IHC staining for nuclear, cytoplasmic, and membrane Notch-1 (N1), Notch-4 (N4), and Jagged-1 (JAG1) was performed and correlated wi...

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Bibliographic Details
Published in:International journal of surgical pathology 2011-10, Vol.19 (5), p.607-613
Main Authors: Yao, Katharine, Rizzo, Paola, Rajan, Prabha, Albain, Kathy, Rychlik, Karen, Shah, Sneha, Miele, Lucio
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - analysis
Breast Neoplasms - metabolism
Breast Neoplasms - mortality
Breast Neoplasms - pathology
Calcium-Binding Proteins - analysis
Calcium-Binding Proteins - biosynthesis
Female
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins - analysis
Intercellular Signaling Peptides and Proteins - biosynthesis
Jagged-1 Protein
Membrane Proteins - analysis
Membrane Proteins - biosynthesis
Middle Aged
Neoplasm Grading
Neoplasm Staging
Prognosis
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins - biosynthesis
Receptor, Notch1 - analysis
Receptor, Notch1 - biosynthesis
Receptor, Notch4
Receptors, Notch - analysis
Receptors, Notch - biosynthesis
Serrate-Jagged Proteins
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Summary:Background: Studies looking at immunohistochemical (IHC) staining of Notch receptors in breast cancer and correlation with known prognostic factors are sparse. Methods: IHC staining for nuclear, cytoplasmic, and membrane Notch-1 (N1), Notch-4 (N4), and Jagged-1 (JAG1) was performed and correlated with known prognostic factors. Results: Of 48 breast cancers, 36 (67%) were invasive, mean age was 50 years (range 43-86 years), 37 (77%) were estrogen receptor (ERα) positive, and 13 (27%) node positive. There was significantly more marked N1 membranous staining in ERα-positive tumors (P < .05). On univariate analysis, cytoplasmic N1 was significantly correlated with node status and tumor grade (P < .05); both cytoplasmic and membranous N4 significantly correlated with Ki67 (P < .05); and membranous JAG1 significantly correlated with Ki67 (P < .05). On multivariate analysis, only cytoplasmic N1 significantly correlated with node status. Conclusion: IHC of Notch markers is feasible and correlates with known prognostic factors consistent with a biological role of Notch signaling in breast cancer progression.
ISSN:1066-8969
1940-2465
DOI:10.1177/1066896910362080