An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells

A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-11, Vol.59 (22), p.12193-12197
Main Authors: Vo, Thanh-Sang, Ngo, Dai-Hung, Kim, Jung-Ae, Ryu, BoMi, Kim, Se-Kwon
Format: Article
Language:English
Subjects:
Animals
Antihypertensive Agents - chemistry
Antihypertensive Agents - metabolism
Antihypertensive Agents - pharmacology
antihypertensive effect
Biological and medical sciences
DNA damage
DNA Damage - drug effects
Fish Proteins - chemistry
Fish Proteins - metabolism
Fish Proteins - pharmacology
Food Chemistry/Biochemistry
Food industries
foods
Free Radicals - metabolism
Fundamental and applied biological sciences. Psychology
gelatin
Gelatin - chemistry
Gelatin - metabolism
Gelatin - pharmacology
Humans
hydrolysates
Hydrolysis
hypertension
Hypertension - metabolism
Mice
Microglia - drug effects
Microglia - metabolism
Oreochromis niloticus
oxidative stress
pepsin
Peptides - metabolism
Peptides - pharmacology
peptidyl-dipeptidase A
protective effect
subtilisin
Tilapia - metabolism
trypsin
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Summary:A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf202837g