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An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells
A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further...
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| Published in: | Journal of agricultural and food chemistry 2011-11, Vol.59 (22), p.12193-12197 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a368t-4c71e09654567164fab0372361cf808ef002bfac446e6efae75bb0d524dfe7ab3 |
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| cites | cdi_FETCH-LOGICAL-a368t-4c71e09654567164fab0372361cf808ef002bfac446e6efae75bb0d524dfe7ab3 |
| container_end_page | 12197 |
| container_issue | 22 |
| container_start_page | 12193 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 59 |
| creator | Vo, Thanh-Sang Ngo, Dai-Hung Kim, Jung-Ae Ryu, BoMi Kim, Se-Kwon |
| description | A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods. |
| doi_str_mv | 10.1021/jf202837g |
| format | article |
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Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf202837g</identifier><identifier>PMID: 22004328</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Antihypertensive Agents - chemistry ; Antihypertensive Agents - metabolism ; Antihypertensive Agents - pharmacology ; antihypertensive effect ; Biological and medical sciences ; DNA damage ; DNA Damage - drug effects ; Fish Proteins - chemistry ; Fish Proteins - metabolism ; Fish Proteins - pharmacology ; Food Chemistry/Biochemistry ; Food industries ; foods ; Free Radicals - metabolism ; Fundamental and applied biological sciences. Psychology ; gelatin ; Gelatin - chemistry ; Gelatin - metabolism ; Gelatin - pharmacology ; Humans ; hydrolysates ; Hydrolysis ; hypertension ; Hypertension - metabolism ; Mice ; Microglia - drug effects ; Microglia - metabolism ; Oreochromis niloticus ; oxidative stress ; pepsin ; Peptides - metabolism ; Peptides - pharmacology ; peptidyl-dipeptidase A ; protective effect ; subtilisin ; Tilapia - metabolism ; trypsin</subject><ispartof>Journal of agricultural and food chemistry, 2011-11, Vol.59 (22), p.12193-12197</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a368t-4c71e09654567164fab0372361cf808ef002bfac446e6efae75bb0d524dfe7ab3</citedby><cites>FETCH-LOGICAL-a368t-4c71e09654567164fab0372361cf808ef002bfac446e6efae75bb0d524dfe7ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24771806$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22004328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vo, Thanh-Sang</creatorcontrib><creatorcontrib>Ngo, Dai-Hung</creatorcontrib><creatorcontrib>Kim, Jung-Ae</creatorcontrib><creatorcontrib>Ryu, BoMi</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><title>An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.</description><subject>Animals</subject><subject>Antihypertensive Agents - chemistry</subject><subject>Antihypertensive Agents - metabolism</subject><subject>Antihypertensive Agents - pharmacology</subject><subject>antihypertensive effect</subject><subject>Biological and medical sciences</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Fish Proteins - chemistry</subject><subject>Fish Proteins - metabolism</subject><subject>Fish Proteins - pharmacology</subject><subject>Food Chemistry/Biochemistry</subject><subject>Food industries</subject><subject>foods</subject><subject>Free Radicals - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gelatin</subject><subject>Gelatin - chemistry</subject><subject>Gelatin - metabolism</subject><subject>Gelatin - pharmacology</subject><subject>Humans</subject><subject>hydrolysates</subject><subject>Hydrolysis</subject><subject>hypertension</subject><subject>Hypertension - metabolism</subject><subject>Mice</subject><subject>Microglia - drug effects</subject><subject>Microglia - metabolism</subject><subject>Oreochromis niloticus</subject><subject>oxidative stress</subject><subject>pepsin</subject><subject>Peptides - metabolism</subject><subject>Peptides - pharmacology</subject><subject>peptidyl-dipeptidase A</subject><subject>protective effect</subject><subject>subtilisin</subject><subject>Tilapia - metabolism</subject><subject>trypsin</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpt0U1v1DAQBmALUdGlcOAPgC8IcUgZO_7KcbWwpVIrELTnyEnGW68SJ7UTpP77Gu3SXjj5MI9Gr98h5B2Dcwacfdk7DtyUeveCrJjkUEjGzEuygjwsjFTslLxOaQ8ARmp4RU45BxAlNysyrANdh9nfPUwYZwzJ_0H6E6fZd0hdHAd643s7eUsvsLezD_SrH3zw6Q4T3UZE-st2vrU93Y5xyGAMNKPrJfqA9Nq3cdz1Po832PfpDTlxtk_49viekdvtt5vN9-Lqx8XlZn1V2FKZuRCtZgiVkkIqzZRwtoFS81Kx1hkw6PLHGmdbIRQqdBa1bBroJBedQ22b8ox8Ouyd4ni_YJrrwac2J7ABxyXVFUilKqmqLD8fZA6aUkRXT9EPNj7UDOq_5dZP5Wb7_rh1aQbsnuS_NjP4eAQ25UpctKH16dkJrZkBld2Hg3N2rO0uZnP7mwMT-UKVKoV-3mTbVO_HJYZc138iPQIGaZcE</recordid><startdate>20111123</startdate><enddate>20111123</enddate><creator>Vo, Thanh-Sang</creator><creator>Ngo, Dai-Hung</creator><creator>Kim, Jung-Ae</creator><creator>Ryu, BoMi</creator><creator>Kim, Se-Kwon</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111123</creationdate><title>An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells</title><author>Vo, Thanh-Sang ; Ngo, Dai-Hung ; Kim, Jung-Ae ; Ryu, BoMi ; Kim, Se-Kwon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a368t-4c71e09654567164fab0372361cf808ef002bfac446e6efae75bb0d524dfe7ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antihypertensive Agents - chemistry</topic><topic>Antihypertensive Agents - metabolism</topic><topic>Antihypertensive Agents - pharmacology</topic><topic>antihypertensive effect</topic><topic>Biological and medical sciences</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>Fish Proteins - chemistry</topic><topic>Fish Proteins - metabolism</topic><topic>Fish Proteins - pharmacology</topic><topic>Food Chemistry/Biochemistry</topic><topic>Food industries</topic><topic>foods</topic><topic>Free Radicals - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gelatin</topic><topic>Gelatin - chemistry</topic><topic>Gelatin - metabolism</topic><topic>Gelatin - pharmacology</topic><topic>Humans</topic><topic>hydrolysates</topic><topic>Hydrolysis</topic><topic>hypertension</topic><topic>Hypertension - metabolism</topic><topic>Mice</topic><topic>Microglia - drug effects</topic><topic>Microglia - metabolism</topic><topic>Oreochromis niloticus</topic><topic>oxidative stress</topic><topic>pepsin</topic><topic>Peptides - metabolism</topic><topic>Peptides - pharmacology</topic><topic>peptidyl-dipeptidase A</topic><topic>protective effect</topic><topic>subtilisin</topic><topic>Tilapia - metabolism</topic><topic>trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vo, Thanh-Sang</creatorcontrib><creatorcontrib>Ngo, Dai-Hung</creatorcontrib><creatorcontrib>Kim, Jung-Ae</creatorcontrib><creatorcontrib>Ryu, BoMi</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vo, Thanh-Sang</au><au>Ngo, Dai-Hung</au><au>Kim, Jung-Ae</au><au>Ryu, BoMi</au><au>Kim, Se-Kwon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2011-11-23</date><risdate>2011</risdate><volume>59</volume><issue>22</issue><spage>12193</spage><epage>12197</epage><pages>12193-12197</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A peptide possessing antihypertensive activity was purified from Nile tilapia (Oreochromis niloticus) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22004328</pmid><doi>10.1021/jf202837g</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2011-11, Vol.59 (22), p.12193-12197 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Animals Antihypertensive Agents - chemistry Antihypertensive Agents - metabolism Antihypertensive Agents - pharmacology antihypertensive effect Biological and medical sciences DNA damage DNA Damage - drug effects Fish Proteins - chemistry Fish Proteins - metabolism Fish Proteins - pharmacology Food Chemistry/Biochemistry Food industries foods Free Radicals - metabolism Fundamental and applied biological sciences. Psychology gelatin Gelatin - chemistry Gelatin - metabolism Gelatin - pharmacology Humans hydrolysates Hydrolysis hypertension Hypertension - metabolism Mice Microglia - drug effects Microglia - metabolism Oreochromis niloticus oxidative stress pepsin Peptides - metabolism Peptides - pharmacology peptidyl-dipeptidase A protective effect subtilisin Tilapia - metabolism trypsin |
| title | An Antihypertensive Peptide from Tilapia Gelatin Diminishes Free Radical Formation in Murine Microglial Cells |
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