Structural characterization of the O-chain polysaccharide from an environmentally beneficial bacterium Pseudomonas chlororaphis subsp. aureofaciens strain M71

Pseudomonas chlororaphis subsp. aureofaciens strain M71 was isolated from the root of a tomato plant and it was able to control in vivo Fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. Recently, strain M71 was evaluated even for its efficacy in controlling...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 2011-12, Vol.346 (17), p.2705-2709
Main Authors: Pieretti, Giuseppina, Puopolo, Gerardo, Carillo, Sara, Zoina, Astolfo, Lanzetta, Rosa, Parrilli, Michelangelo, Evidente, Antonio, Corsaro, Maria Michela
Format: Article
Language:English
Subjects:
acid hydrolysis
Antibiosis
bacteria
bark
Biological control
Carbohydrate Conformation
Carbohydrate Sequence
Cupressus sempervirens
Fusarium oxysporum
Gas Chromatography-Mass Spectrometry
Lipopolysaccharide
lipopolysaccharides
Magnetic Resonance Spectroscopy
Molecular Sequence Data
NMR spectroscopy
nuclear magnetic resonance spectroscopy
O Antigens - chemistry
O Antigens - isolation & purification
O-Specific polysaccharide
Plant Diseases - microbiology
polymers
polysaccharides
Pseudomonas
Pseudomonas chlororaphis
Pseudomonas chlororaphis subsp. aureofaciens
rhizosphere
root crown
root rot
Seiridium cardinale
tomatoes
trisaccharides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pseudomonas chlororaphis subsp. aureofaciens strain M71 was isolated from the root of a tomato plant and it was able to control in vivo Fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. Recently, strain M71 was evaluated even for its efficacy in controlling Seiridium cardinale, the causal agent of bark canker of common cypress ( Cupressus sempervirens L.). Strain M71 ability to persist on the tomato rhizosphere and on the aerial part of cypress plants could be related to the nature of the lipopolysaccharides (LPS) present on the outer membrane and in particular to the O-specific polysaccharide. A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide from P. chlororaphis subsp. aureofaciens strain M71. By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as the following linear trisaccharide. ▪
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2011.09.027