Knockdown of Rad51 expression induces radiation- and chemo-sensitivity in osteosarcoma cells

• Published in: Medical oncology (Northwood, London, England) London, England), 2011-12, Vol.28 (4), p.1481-1487
• Main Authors: Du, Li-Qing, Wang, Yong, Wang, Hong, Cao, Jia, Liu, Qiang, Fan, Fei-Yue
• Format: Article
• Language: English
• Subjects: Animals; Blotting, Western; Bone; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Cell cycle; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Separation; Children; DNA damage; DNA repair; Drug Resistance, Neoplasm - genetics; Female; Flow Cytometry; G2 phase; Gene Knockdown Techniques; Hematology; homologous recombination; Humans; Internal Medicine; Ionizing radiation; Male; Malignancy; Medicine; Medicine & Public Health; Mice; Mice, Nude; Oncology; Original Paper; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma cells; Pathology; Plasmids; Rad51 Recombinase - biosynthesis; Rad51 Recombinase - genetics; Radiation Tolerance - genetics; Real-Time Polymerase Chain Reaction; RNA; RNA, Small Interfering; Transfection; Transplantation, Heterologous; Tumor cells
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-010-9605-1

Osteosarcoma is the common primary bone malignancy in children and young adults in Eastern countries. Resistance to ionizing radiation (IR) or drugs is an underlying mechanism contributing to the failure of therapy in these patients. Rad51 is the key protein of DNA homologous recombination repair. Although high expression of Rad51 is associated with enhanced resistance to DNA damage induced by chemicals and/or ionizing radiation, the relevance of Rad51 expression in osteosarcoma and its relationship with IR sensitivity and chemo-resistance is not well understood. In this study, we elucidated the possibility of using Rad51 in the treatment of human osteosarcoma in vitro. Changes in chemo- and radiation sensitivity in cultured osteosarcoma cells occurred after suppression of Rad51 expression, using a plasmid vector-mediated short hairpin RNA (shRNA) expression system. The suppression of Rad51 correlated with cell cycle arrest in the G 2 phase and inhibited tumor cell proliferation. Our results suggest that Rad51 expression levels might play an important role in radiation- and chemo-sensitivity of human osteosarcoma.