New insights into RNA packaging in porcine reproductive and respiratory syndrome virus

While identifying whether the smallest packaged heteroclite subgenomic RNA (S9) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a packaging signal, we found that S9 was capable of binding to the basic amino acid-rich domain (synthetic peptide of aa 34-53) of the packaging pro...

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Bibliographic Details
Published in:Journal of general virology 2011-12, Vol.92 (Pt 12), p.2865-2870
Main Authors: BAIG, Tayyba T, ZAKHARTCHOUK, Alexander
Format: Article
Language:English
Subjects:
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Animals
Biological and medical sciences
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Genetic Markers
Microbiology
Miscellaneous
Porcine respiratory and reproductive syndrome virus - genetics
Porcine respiratory and reproductive syndrome virus - metabolism
Porcine respiratory and reproductive syndrome virus - physiology
Protein Binding
RNA, Viral - genetics
RNA, Viral - metabolism
Sequence Analysis, RNA - methods
Swine - virology
Virology
Virus Assembly
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Summary:While identifying whether the smallest packaged heteroclite subgenomic RNA (S9) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a packaging signal, we found that S9 was capable of binding to the basic amino acid-rich domain (synthetic peptide of aa 34-53) of the packaging protein (N). In addition, by using truncations at the 5' and 3' ends of S9, a minimal binding region of 35 nt was found to be essential for binding to both the synthetic peptide and to the full-length N protein. Furthermore, by using cell-culture experiments, we found that S9 was capable of packaging non-viral RNA sequence into PRRSV particles and that the 35 nt region was essential for this activity. Taken together, our data suggest that this 35 nt region might be an important element for packaging PRRSV genomic RNA into virus particles.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.036079-0