Study on the role of environmental parameters and HIF-1A gene polymorphism in coronary collateral formation among patients with ischemic heart disease

• Published in: Clinical biochemistry 2011-12, Vol.44 (17), p.1421-1424
• Main Authors: Alidoosti, Mohammad, Ghaedi, Mahboobeh, Soleimani, Abbas, Bakhtiyari, Salar, Rezvanfard, Mehrnaz, Golkhu, Shekufeh, Mohammadtaghvaei, Narges
• Format: Article
• Language: English
• Subjects: Aged; Amino Acid Substitution; Biological and medical sciences; Cardiology. Vascular system; Collateral Circulation - genetics; Coronary Angiography; Coronary collateral formation; Coronary heart disease; Environmental Exposure - adverse effects; Female; Genetic Association Studies; Genotype; Heart; Humans; Hypoxia-inducible factor 1 (HIF-1); Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Investigative techniques, diagnostic techniques (general aspects); Iranian population; Male; Medical sciences; Middle Aged; Multivariate Analysis; Myocardial Ischemia - diagnostic imaging; Myocardial Ischemia - etiology; Myocardial Ischemia - genetics; Myocarditis. Cardiomyopathies; Neovascularization, Physiologic - genetics; Polymorphism; Polymorphism, Single Nucleotide; Sequence Analysis, DNA
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2011.09.001

To evaluate the association between collateral formation and some environmental factors along with a polymorphism in HIF-1A gene in selected Iranian patients with CAD. Patients with ≥ 70% narrowing in at least one coronary vessel according to coronary angiography were enrolled. The patients' demographic, clinical and biochemical data were collected. The presence of C1772T polymorphisms within HIF-1A was analyzed using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). There is no significant difference between the patients with and without collaterals according to the frequency of T allele or HIF-1A variants. The higher severity of coronary vessel obstruction was positive predictor of collateral formation (OR = 1.026, 95%, CI: 1.02–0.04, p < 0.001), whereas aging and cigarette smoking were negative predictors (OR = 0.95, 95% CI: 0.91–0.99, p < 0.05; OR = 0.30, 95% CI: 0.11–0.79, p < 0.05; respectively). The findings indicate not any significant association between collateral formation and polymorphic variants of HIF-1A and P582S substitution does not appear to influence the collateral formation in patients with myocardial ischemia