Molecular identification of a causative parasite species using formalin-fixed paraffin embedded (FFPE) tissues of a complicated human pulmonary sparganosis case without decisive clinical diagnosis

Abstract PCR-based molecular diagnosis was made for the identification of causative agents of the clinically suspected pulmonary proliferative sparganosis case found in Thailand using formalin-fixed paraffin-embedded (FFPE) biopsy specimens. As a reference, FFPE biopsy specimen from a typical cutane...

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Bibliographic Details
Published in:Parasitology international 2011-12, Vol.60 (4), p.460-464
Main Authors: Koonmee, Supinda, Intapan, Pewpan M, Yamasaki, Hiroshi, Sugiyama, Hiromu, Muto, Maki, Kuramochi, Toshiaki, Kularbkeaw, Jurairat, Kanpittaya, Jaturat, Maleewong, Wanchai, Nawa, Yukifumi
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Base Sequence
Biopsy
cox1
cytochrome-c oxidase
DNA
DNA, Helminth - analysis
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - genetics
Fatal Outcome
Gastroenterology and Hepatology
genes
Humans
Infectious Disease
Lung - parasitology
Lung - physiopathology
Male
Microtomy
Molecular diagnosis
Molecular Sequence Data
nucleotide sequences
Paraffin Embedding
Paraffin section
parasites
parasitology
PCR
Polymerase Chain Reaction
Protein Subunits - chemistry
Protein Subunits - genetics
Sequence Analysis, DNA
Sparganosis
Sparganosis - diagnosis
Sparganosis - parasitology
Sparganosis - physiopathology
Sparganum - growth & development
Sparganum - ultrastructure
Spirometra
Spirometra erinaceieuropaei
Thailand
Tissue Fixation
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Summary:Abstract PCR-based molecular diagnosis was made for the identification of causative agents of the clinically suspected pulmonary proliferative sparganosis case found in Thailand using formalin-fixed paraffin-embedded (FFPE) biopsy specimens. As a reference, FFPE biopsy specimen from a typical cutaneous sparganosis case was examined together. DNA samples were extracted from tissues and two partial fragments of cytochrome c oxidase subunit 1 ( cox1 ) gene were amplified for the detection of Spirometra DNA. Two cox1 fragments were amplified successfully for both specimens. After alignment of nucleotide sequences of the PCR-amplicons, the causative agents of both cases were identified as Spirometra erinaceieuropaei.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2011.07.018