Porphyrins in urine after administration of 5-aminolevulinic acid as a potential tumor marker

Summary Background Tumor markers are commonly used for cancer screening and as indicators of therapeutic effects. Certain types of tumor have been known to produce a variety of porphyrins after 5-aminolevulinic acid (ALA) administration. In this study, porphyrins in tumor-bearing mouse urine were an...

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Bibliographic Details
Published in:Photodiagnosis and photodynamic therapy 2011-12, Vol.8 (4), p.328-331
Main Authors: Ishizuka, Masahiro, Hagiya, Yuichiro, Mizokami, Yasuhiro, Honda, Kanako, Tabata, Kenji, Kamachi, Toshiaki, Takahashi, Kiwamu, Abe, Fuminori, Tanaka, Tohru, Nakajima, Motowo, Ogura, Shun-ichiro, PhD, Okura, Ichiro
Format: Article
Language:English
Subjects:
Aminolevulinic acid
Aminolevulinic Acid - administration & dosage
Animals
Biomarkers, Tumor - urine
Carcinoma, Hepatocellular - diagnosis
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - urine
Cell Line, Tumor
Coproporphyrin
Hematology, Oncology and Palliative Medicine
Internal Medicine
Mice
Mice, Inbred C3H
Photochemotherapy - methods
Photosensitizing Agents - administration & dosage
Porphyrins - urine
Reproducibility of Results
Sensitivity and Specificity
Treatment Outcome
Tumor marker
Urine
Uroporphyrin
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Summary:Summary Background Tumor markers are commonly used for cancer screening and as indicators of therapeutic effects. Certain types of tumor have been known to produce a variety of porphyrins after 5-aminolevulinic acid (ALA) administration. In this study, porphyrins in tumor-bearing mouse urine were analyzed after oral administration of ALA in order to identify new tumor markers excreted in the urine. Methods Porphyrin concentrations in the urine of tumor-bearing mice were measured after administration of 1.0 mg of ALA (approximately 50 mg kg−1 ). Results Porphyrin concentrations in the urine of tumor-bearing mice increased after administration of ALA. HPLC analysis of the urine revealed the existence of uroporphyrin (UP) and coproporphyrin (CP) in the urine of ALA-treated tumor-bearing mice. Furthermore, at 3 h after ALA administration, UP concentrations in the urine of tumor-bearing mice significantly increased compared to those in the urine of normal mice. Conclusion These results suggest that UP as a precursor of heme detected in the urine of tumor-bearing mice after ALA administration is a potential marker of tumor development.
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2011.04.004