Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein

We have developed a nanotube-based colorimetric probe using multiwalled carbon nanotubes (MWNTs), anti-immunoglobulin G (anti-IgG), and horseradish peroxidase (HRP). The probe was used as an alternative to conventional colorimetric conjugates to obtain amplified signals in a sandwich-type immunoassa...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2011-12, Vol.83 (23), p.9191-9196
Main Authors: Zhang, Qingzhi, Zhao, Bin, Yan, Juan, Song, Shiping, Min, Rui, Fan, Chunhai
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Ataxia Telangiectasia Mutated Proteins
Biomarkers
Biomarkers, Tumor - analysis
Biomarkers, Tumor - immunology
Cell Cycle Proteins - analysis
Cell Cycle Proteins - immunology
Chemistry
Colorimetry
DNA-Binding Proteins - analysis
DNA-Binding Proteins - immunology
Exact sciences and technology
Horseradish Peroxidase - metabolism
Immunoassay
Immunoglobulin G - immunology
Measurement techniques
Miscellaneous
Nanotubes
Nanotubes, Carbon - chemistry
Neurodegeneration
Protein-Serine-Threonine Kinases - analysis
Protein-Serine-Threonine Kinases - immunology
Proteins
Rabbits
Spectrometric and optical methods
Tumor Suppressor Proteins - analysis
Tumor Suppressor Proteins - immunology
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Summary:We have developed a nanotube-based colorimetric probe using multiwalled carbon nanotubes (MWNTs), anti-immunoglobulin G (anti-IgG), and horseradish peroxidase (HRP). The probe was used as an alternative to conventional colorimetric conjugates to obtain amplified signals in a sandwich-type immunoassay for ataxia telangiectasia mutated (ATM), a potential biomarker for radiation doses and cancers. Results show that the MWNT-based probe colorimetry was 5000 times more sensitive than a conventional ELISA, while its concentration range was 10 000 times wider than that of the latter. Its limit of detection (LOD) was 0.2 fg/mL (54 aM, ∼32 molecules in 1 μL samples). Control experiments showed that detection of ATM molecules at the picogram-level could still be achieved in samples that contained protein makers present at more than 100 times the ATM concentration, demonstrating the high specificity of the technique. The MWNT-based probe also has the potential to become a universal probe for colorimetric assays of most protein markers because it can recognize the associated rabbit polyclonal antibodies.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac2023684