A single polymerase (L) mutation in avian metapneumovirus increased virulence and partially maintained virus viability at an elevated temperature

Previously, a virulent avian metapneumovirus, farm isolate Italy 309/04, was shown to have been derived from a live vaccine. Virulence due to the five nucleotide mutations associated with the reversion to virulence was investigated by their addition to the genome of the vaccine strain using reverse...

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Bibliographic Details
Published in:Journal of general virology 2011-02, Vol.92 (Pt 2), p.346-354
Main Authors: BROWN, Paul A, LUPINI, Caterina, CATELLI, Elena, CLUBBE, Jayne, RICCHIZZI, Enrico, NAYLOR, Clive J
Format: Article
Language:English
Subjects:
Animals
Avian metapneumovirus
Biological and medical sciences
Cercopithecus aethiops
Cloning, Molecular
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Viral
Hot Temperature
Metapneumovirus - genetics
Metapneumovirus - metabolism
Metapneumovirus - pathogenicity
Microbial Viability
Microbiology
Miscellaneous
Mutation
Paramyxoviridae Infections - veterinary
Paramyxoviridae Infections - virology
Plasmids
Poultry Diseases - virology
Time Factors
Turkeys
Vero Cells
Virology
Virulence
Virus Replication
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Summary:Previously, a virulent avian metapneumovirus, farm isolate Italy 309/04, was shown to have been derived from a live vaccine. Virulence due to the five nucleotide mutations associated with the reversion to virulence was investigated by their addition to the genome of the vaccine strain using reverse genetics. Virulence of these recombinant viruses was determined by infection of 1-day-old turkeys. Disease levels resulting from the combined two matrix mutations was indistinguishable from that produced by the recombinant vaccine, whereas the combined three L gene mutations increased disease to a level (P
ISSN:0022-1317
1465-2099
1465-2099
DOI:10.1099/vir.0.026740-0