Transmitter Sensitivity of Primary Afferent Cells of the Lamprey Lampetra fluviatilis Spinal Cord

• Published in: Journal of evolutionary biochemistry and physiology 2002-01, Vol.38 (1), p.57-64
• Main Authors: Batueva, I V, Buchanan, J T, Vesselkin, N P, Suderevskaya, E I, Tsvetkov, E A
• Format: Article
• Language: English
• Subjects: Amino acids; Lampetra fluviatilis; Petromyzontidae
• ISSN: 0022-0930; 1608-3202
• DOI: 10.1023/A:1015517321429

Using dorsal sensory cells (primary afferents) of the spinal cord of the lamprey Lampetra fluviatilis, isolated by an enzymatic-mechanical method, their responses have been studied to application of excitatory amino acids (EAA), glutamate, aspartate, kainate, N-methyl-D-aspartate (NMDA), and of inhibitory amino acids, GABA and glycine, as well as of neuromodulator serotonin (5-hydroxytryptamine, 5-HT). The patch-clamp method was applied to fix either potential or current through membranes of studied cells. It was shown that of all the above substances, only NMDA and kainate could produce in dorsal cells depolarization, input current with maximal amplitude up to 16 nA, and action potential. The main attention in this work was paid to NMDA-current properties. The dose-effect curves were obtained; a blocking effect of specific antagonist of NMDA-receptors, 2-amino-5-phosphonovalerian acid (APV), was shown; passage of Ba ions through Ca^sup 2+^-channels of dorsal sensory cell membranes at NMDA application was proven. It has been established that in a half of the studied cells under effect of NMDA there is an increase of the maximal amplitude of the potential-activated current through Ca^sup 2+^-channels, on average, by 22.5 ± 10.5% (n = 21). It was suggested that this variation might be essential for an increase of amount of the transmitter released in synapses formed by processes dorsal sensory cells, while NMDA could be considered a modulator facilitating synaptic activity of these cells.[PUBLICATION ABSTRACT]