Multilocus phylogenetic analysis of the genus Aeromonas

A broad multilocus phylogenetic analysis (MLPA) of the representative diversity of a genus offers the opportunity to incorporate concatenated inter-species phylogenies into bacterial systematics. Recent analyses based on single housekeeping genes have provided coherent phylogenies of Aeromonas. Howe...

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Bibliographic Details
Published in:Systematic and applied microbiology 2011-05, Vol.34 (3), p.189-199
Main Authors: Martinez-Murcia, Antonio J., Monera, Arturo, Saavedra, M. Jose, Oncina, Remedios, Lopez-Alvarez, Monserrate, Lara, Erica, Figueras, M. Jose
Format: Article
Language:English
Subjects:
Aeromonas
Aeromonas - classification
Aeromonas - genetics
atpD
Bacterial Typing Techniques
Base Sequence
Biological and medical sciences
DNA topoisomerase
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
dnaJ
dnaX
Fundamental and applied biological sciences. Psychology
Genes, Bacterial - genetics
gyrA
gyrB
Microbiology
MLPA
Molecular Sequence Data
Multilocus
Multilocus Sequence Typing - methods
Phylogeny
recA
RecA protein
rpoD
Sequence Alignment
Sequence Analysis, DNA
Taxonomy
Trees
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Summary:A broad multilocus phylogenetic analysis (MLPA) of the representative diversity of a genus offers the opportunity to incorporate concatenated inter-species phylogenies into bacterial systematics. Recent analyses based on single housekeeping genes have provided coherent phylogenies of Aeromonas. However, to date, a multi-gene phylogenetic analysis has never been tackled. In the present study, the intra- and inter-species phylogenetic relationships of 115 strains representing all Aeromonas species described to date were investigated by MLPA. The study included the independent analysis of seven single gene fragments ( gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD), and the tree resulting from the concatenated 4705 bp sequence. The phylogenies obtained were consistent with each other, and clustering agreed with the Aeromonas taxonomy recognized to date. The highest clustering robustness was found for the concatenated tree (i.e. all Aeromonas species split into 100% bootstrap clusters). Both possible chronometric distortions and poor resolution encountered when using single-gene analysis were buffered in the concatenated MLPA tree. However, reliable phylogenetic species delineation required an MLPA including several “bona fide” strains representing all described species.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2010.11.014