Salmonella Serovars and Antimicrobial Resistance Profiles in Beef Cattle, Slaughterhouse Personnel and Slaughterhouse Environment in Ethiopia

• Published in: Zoonoses and public health 2011-03, Vol.58 (2), p.102-109
• Main Authors: Sibhat, B, Molla Zewde, B, Zerihun, A, Muckle, A, Cole, L, Boerlin, P, Wilkie, E, Perets, A, Mistry, K, Gebreyes, W.A
• Format: Article
• Language: English
• Subjects: Abattoirs - manpower; Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; antimicrobial resistance; Cattle; Cattle - microbiology; Colony Count, Microbial; Consumer Product Safety; Drug resistance; Drug Resistance, Bacterial; Environmental Microbiology; Ethiopia; Ethiopia - epidemiology; Food Microbiology; Human exposure; Humans; Meat processing; Microbial Sensitivity Tests; Occupational health; prevalence; Salmonella; Salmonella - classification; Salmonella - drug effects; Salmonella - isolation & purification; Salmonella Infections, Animal - drug therapy; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - transmission; Serotyping; serovars; Slaughter cattle; Zoonoses
• ISSN: 1863-1959; 1863-2378
• DOI: 10.1111/j.1863-2378.2009.01305.x

The present study was undertaken to determine the occurrence, distribution and antimicrobial resistance profiles of Salmonella serovars in slaughter beef cattle, slaughterhouse environment and personnel engaged in flaying and evisceration during slaughtering process. A total of 800 samples (each sample type, n = 100) consisting of swabs from hides, slaughterhouse personnel hands at flaying and evisceration, rumen and caecal contents, mesenteric lymph nodes, carcasses and holding pens were collected. Of the total 100 beef cattle examined, 14% were Salmonella positive in caecal content and/or mesenteric lymph nodes. Of the various samples analysed, Salmonella was detected in 31% of hides, 19% of rumen contents, 8% of mesenteric lymph nodes, 6% of caecal contents, 2% of carcass swabs, 9% of palm swabs taken from the hands of personnel in the slaughterhouse during flaying (7%) and evisceration (2%), and in 12% of holding pen swabs. The Salmonella isolates (n = 87) belonged to eight different serovars of which S. Anatum (n = 54) and S. Newport (19) were the major serovars and both serovars were detected in all sample sources except in carcass swabs. Eighteen of the 87 (20.7%) Salmonella serovars consisting of Newport (n = 14), Anatum (n = 3) and Eastbourne (n = 1) were resistant to one or more antimicrobials. Among the antimicrobial resistant Salmonella serovars, S. Newport was multidrug resistant (15.6%) and exhibited resistance to streptomycin, sulphisoxazole and tetracycline.