Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent extract of HeLa cells. Synthesis and application of long model DNA

Long linear DNA analogs of nucleotide excision repair (NER) substrates have been synthesized. They are 137-mer duplexes containing in their internal positions nucleotides with bulky substitutes imitating lesions with fluorochloroazidopyridyl and fluorescein groups introduced using spacer fragments a...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2011, Vol.76 (1), p.157-166
Main Authors: Evdokimov, A. N, Petruseva, I. O, Pestryakov, P. E, Lavrik, O. I
Format: Article
Language:English
Subjects:
Base Sequence
Binding sites
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Deoxyribonucleic acid
DNA
DNA - chemical synthesis
DNA - chemistry
DNA - genetics
DNA Damage
DNA Repair
DNA Repair Enzymes - genetics
DNA Repair Enzymes - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
HeLa Cells
Humans
Lesions
Life Sciences
Microbiology
Molecular Sequence Data
Nucleotides
Photochemistry
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases - genetics
Poly(ADP-ribose) Polymerases - metabolism
Protein Binding
Proteins
Substrates
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Summary:Long linear DNA analogs of nucleotide excision repair (NER) substrates have been synthesized. They are 137-mer duplexes containing in their internal positions nucleotides with bulky substitutes imitating lesions with fluorochloroazidopyridyl and fluorescein groups introduced using spacer fragments at the 4N and 5C positions of dCMP and dUMP (Fap-dC- and Flu-dU-DNA) and DNA containing a (+)-cis-stereoisomer of benzo[a]pyrene-N2-deoxyguanidine (BP-dG-DNA, 131 bp). The interaction of the modified DNA duplexes with the proteins of NER-competent HeLa extract was investigated. The substrate properties of the model DNA in the reaction of specific excision were shown to vary in the series Fap-dC-DNA
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297911010159