Thyroid-stimulating hormone (TSH): Measurement of intracellular, secreted, and circulating hormone in Xenopus laevis and Xenopus tropicalis

Highlights ► Sensitive and specific measurement of TSH in Xenopus laevis and Xenopus tropicalis . ► Ability to measure intracellular, secreted, and circulating TSH. ► First report for direct measurement of circulating TSH in amphibians. ► Purification of X. laevis pituitary TSH.

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Bibliographic Details
Published in:General and comparative endocrinology 2011-05, Vol.171 (3), p.319-325
Main Authors: Korte, Joseph J, Sternberg, Robin M, Serrano, Jose A, Thoemke, Kara R, Moen, Scott M, Lillegard, Kathryn E, Hornung, Michael W, Tietge, Joseph E, Degitz, Sigmund J
Format: Article
Language:English
Subjects:
amino acid sequences
amphibians
Animals
antibodies
blood serum
Blotting, Western
detection limit
Electrophoresis, Polyacrylamide Gel
ELISA
Endocrinology & Metabolism
Enzyme-Linked Immunosorbent Assay
Pituitary
Pituitary Gland - metabolism
polyacrylamide gel electrophoresis
quantitative analysis
secretion
Serum
tadpoles
thyroid hormones
Thyroid-stimulating hormone (TSH)
thyrotropin
Thyrotropin - blood
Thyrotropin - metabolism
Thyrotropin - secretion
Western blot
Western blotting
Xenopus - blood
Xenopus - metabolism
Xenopus laevis
Xenopus laevis - blood
Xenopus laevis - metabolism
Xenopus tropicalis
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Summary:Highlights ► Sensitive and specific measurement of TSH in Xenopus laevis and Xenopus tropicalis . ► Ability to measure intracellular, secreted, and circulating TSH. ► First report for direct measurement of circulating TSH in amphibians. ► Purification of X. laevis pituitary TSH.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2011.02.017