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Rectal Administration of Lactobacillus casei DG Modifies Flora Composition and Toll-Like Receptor Expression in Colonic Mucosa of Patients with Mild Ulcerative Colitis

Background An imbalance in gut microbiota seems to contribute to the development of chronic inflammatory disorders of the gastrointestinal tract, such as ulcerative colitis (UC). Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intesti...

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Published in:Digestive diseases and sciences 2011-04, Vol.56 (4), p.1178-1187
Main Authors: D’Incà, Renata, Barollo, Michela, Scarpa, Marco, Grillo, Alessia Rosaria, Brun, Paola, Vettorato, Maria Grazia, Castagliuolo, Ignazio, Sturniolo, Giacomo Carlo
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cited_by cdi_FETCH-LOGICAL-c499t-1994b33a4ed24793bdfe817641148d1786e3eb554606a42e18b328363f2865a83
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container_title Digestive diseases and sciences
container_volume 56
creator D’Incà, Renata
Barollo, Michela
Scarpa, Marco
Grillo, Alessia Rosaria
Brun, Paola
Vettorato, Maria Grazia
Castagliuolo, Ignazio
Sturniolo, Giacomo Carlo
description Background An imbalance in gut microbiota seems to contribute to the development of chronic inflammatory disorders of the gastrointestinal tract, such as ulcerative colitis (UC). Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored. Aim To evaluate the effect of Lactobacillus casei ( L. casei ) DG, a probiotic strain, on colonic-associated microbiota, mucosal cytokine balance, and toll-like receptor (TLR) expression. Methods Twenty-six patients with mild left-sided UC were randomly allocated to one of three groups for an 8-week treatment period: the first group of 7 patients received oral 5-aminosalicylic acid (5-ASA) alone, the second group of 8 patients received oral 5-ASA plus oral L. casei DG, and the third group of 11 patients received oral 5-ASA and rectal L. casei DG. Biopsies were collected from the sigmoid region to culture mucosal-associated microbes and to assess cytokine and TLR messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (RT-PCR). Results 5-ASA alone or together with oral L. casei DG failed to affect colonic flora and TLR expression in a significant manner, but when coupled with rectally administered L. casei DG, it modified colonic microbiota by increasing Lactobacillus spp. and reducing Enterobacteriaceae . It also significantly reduced TLR-4 and interleukin (IL)-1β mRNA levels and significantly increased mucosal IL-10. Conclusions Manipulation of mucosal microbiota by L. casei DG and its effects on the mucosal immune system seem to be required to mediate the beneficial activities of probiotics in UC patients.
doi_str_mv 10.1007/s10620-010-1384-1
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Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored. Aim To evaluate the effect of Lactobacillus casei ( L. casei ) DG, a probiotic strain, on colonic-associated microbiota, mucosal cytokine balance, and toll-like receptor (TLR) expression. Methods Twenty-six patients with mild left-sided UC were randomly allocated to one of three groups for an 8-week treatment period: the first group of 7 patients received oral 5-aminosalicylic acid (5-ASA) alone, the second group of 8 patients received oral 5-ASA plus oral L. casei DG, and the third group of 11 patients received oral 5-ASA and rectal L. casei DG. Biopsies were collected from the sigmoid region to culture mucosal-associated microbes and to assess cytokine and TLR messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (RT-PCR). Results 5-ASA alone or together with oral L. casei DG failed to affect colonic flora and TLR expression in a significant manner, but when coupled with rectally administered L. casei DG, it modified colonic microbiota by increasing Lactobacillus spp. and reducing Enterobacteriaceae . It also significantly reduced TLR-4 and interleukin (IL)-1β mRNA levels and significantly increased mucosal IL-10. Conclusions Manipulation of mucosal microbiota by L. casei DG and its effects on the mucosal immune system seem to be required to mediate the beneficial activities of probiotics in UC patients.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/s10620-010-1384-1</identifier><identifier>PMID: 20737210</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Administration, Rectal ; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use ; Biochemistry ; Biological and medical sciences ; Colitis, Ulcerative - drug therapy ; Colitis, Ulcerative - therapy ; Colon - microbiology ; Cytokines ; Enterobacteriaceae ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gastroenterology ; Gastroenterology. Liver. Pancreas. Abdomen ; Gastrointestinal system ; Hepatology ; Humans ; Interleukin-10 - biosynthesis ; Interleukin-1beta - biosynthesis ; Intestinal Mucosa - chemistry ; Intestinal Mucosa - microbiology ; Lactobacillus casei ; Medical sciences ; Medicine ; Medicine &amp; Public Health ; Mesalamine ; Mesalamine - therapeutic use ; Microbiota (Symbiotic organisms) ; Oncology ; Original Article ; Other diseases. Semiology ; Probiotics ; Probiotics - administration &amp; dosage ; RNA ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Toll-Like Receptor 4 - biosynthesis ; Transplant Surgery ; Treatment Outcome ; Ulcerative colitis ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Digestive diseases and sciences, 2011-04, Vol.56 (4), p.1178-1187</ispartof><rights>Springer Science+Business Media, LLC 2010</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2011 Springer</rights><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-1994b33a4ed24793bdfe817641148d1786e3eb554606a42e18b328363f2865a83</citedby><cites>FETCH-LOGICAL-c499t-1994b33a4ed24793bdfe817641148d1786e3eb554606a42e18b328363f2865a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=24095012$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20737210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D’Incà, Renata</creatorcontrib><creatorcontrib>Barollo, Michela</creatorcontrib><creatorcontrib>Scarpa, Marco</creatorcontrib><creatorcontrib>Grillo, Alessia Rosaria</creatorcontrib><creatorcontrib>Brun, Paola</creatorcontrib><creatorcontrib>Vettorato, Maria Grazia</creatorcontrib><creatorcontrib>Castagliuolo, Ignazio</creatorcontrib><creatorcontrib>Sturniolo, Giacomo Carlo</creatorcontrib><title>Rectal Administration of Lactobacillus casei DG Modifies Flora Composition and Toll-Like Receptor Expression in Colonic Mucosa of Patients with Mild Ulcerative Colitis</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><addtitle>Dig Dis Sci</addtitle><description>Background An imbalance in gut microbiota seems to contribute to the development of chronic inflammatory disorders of the gastrointestinal tract, such as ulcerative colitis (UC). Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored. Aim To evaluate the effect of Lactobacillus casei ( L. casei ) DG, a probiotic strain, on colonic-associated microbiota, mucosal cytokine balance, and toll-like receptor (TLR) expression. Methods Twenty-six patients with mild left-sided UC were randomly allocated to one of three groups for an 8-week treatment period: the first group of 7 patients received oral 5-aminosalicylic acid (5-ASA) alone, the second group of 8 patients received oral 5-ASA plus oral L. casei DG, and the third group of 11 patients received oral 5-ASA and rectal L. casei DG. Biopsies were collected from the sigmoid region to culture mucosal-associated microbes and to assess cytokine and TLR messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (RT-PCR). Results 5-ASA alone or together with oral L. casei DG failed to affect colonic flora and TLR expression in a significant manner, but when coupled with rectally administered L. casei DG, it modified colonic microbiota by increasing Lactobacillus spp. and reducing Enterobacteriaceae . It also significantly reduced TLR-4 and interleukin (IL)-1β mRNA levels and significantly increased mucosal IL-10. 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Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored. Aim To evaluate the effect of Lactobacillus casei ( L. casei ) DG, a probiotic strain, on colonic-associated microbiota, mucosal cytokine balance, and toll-like receptor (TLR) expression. Methods Twenty-six patients with mild left-sided UC were randomly allocated to one of three groups for an 8-week treatment period: the first group of 7 patients received oral 5-aminosalicylic acid (5-ASA) alone, the second group of 8 patients received oral 5-ASA plus oral L. casei DG, and the third group of 11 patients received oral 5-ASA and rectal L. casei DG. Biopsies were collected from the sigmoid region to culture mucosal-associated microbes and to assess cytokine and TLR messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (RT-PCR). Results 5-ASA alone or together with oral L. casei DG failed to affect colonic flora and TLR expression in a significant manner, but when coupled with rectally administered L. casei DG, it modified colonic microbiota by increasing Lactobacillus spp. and reducing Enterobacteriaceae . It also significantly reduced TLR-4 and interleukin (IL)-1β mRNA levels and significantly increased mucosal IL-10. Conclusions Manipulation of mucosal microbiota by L. casei DG and its effects on the mucosal immune system seem to be required to mediate the beneficial activities of probiotics in UC patients.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>20737210</pmid><doi>10.1007/s10620-010-1384-1</doi><tpages>10</tpages></addata></record>
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source Springer Nature
subjects Administration, Rectal
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Biochemistry
Biological and medical sciences
Colitis, Ulcerative - drug therapy
Colitis, Ulcerative - therapy
Colon - microbiology
Cytokines
Enterobacteriaceae
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Gastroenterology
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal system
Hepatology
Humans
Interleukin-10 - biosynthesis
Interleukin-1beta - biosynthesis
Intestinal Mucosa - chemistry
Intestinal Mucosa - microbiology
Lactobacillus casei
Medical sciences
Medicine
Medicine & Public Health
Mesalamine
Mesalamine - therapeutic use
Microbiota (Symbiotic organisms)
Oncology
Original Article
Other diseases. Semiology
Probiotics
Probiotics - administration & dosage
RNA
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Toll-Like Receptor 4 - biosynthesis
Transplant Surgery
Treatment Outcome
Ulcerative colitis
Vertebrates: anatomy and physiology, studies on body, several organs or systems
title Rectal Administration of Lactobacillus casei DG Modifies Flora Composition and Toll-Like Receptor Expression in Colonic Mucosa of Patients with Mild Ulcerative Colitis
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