Lactose-Enhanced Cellulase Production by Microbacterium sp. Isolated from Fecal Matter of Zebra (Equus zebra)

• Published in: Current microbiology 2011-03, Vol.62 (3), p.1050-1055
• Main Authors: Sadhu, Sangrila, Saha, Pradipta, Mayilraj, Shanmugam, Maiti, Tushar Kanti
• Format: Article
• Language: English
• Subjects: Actinomycetales - classification; Actinomycetales - isolation & purification; Actinomycetales - metabolism; Animals; Bacteria; Bacterial Typing Techniques; Biomedical and Life Sciences; Biotechnology; Carbohydrate Metabolism; Carbon sources; Cellulase - metabolism; Cellulose; Cluster Analysis; Culture Media - chemistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Enzymes; Equidae; Equus zebra; Feces; Feces - microbiology; Life Sciences; Mammals; Microbacterium; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Synergistic effect; Temperature; Wildlife
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-010-9816-x

A cellulase-producing bacterial strain designated Z5 was isolated from the fecal matter of Zebra ( Equus zebra ). The strain was identified as Microbacterium sp. on the basis of 16S rDNA sequence analysis. The effect of substrates like CMC, avicel, starch, maltose, sucrose, glucose, fructose, galactose, and lactose on cellulase production was also determined. Lactose as the sole carbon source induced cellulase production in this bacterial strain and a positive synergistic effect of lactose and CMC was also observed with enhancement of 3–4 times in cellulase activity. The optimum cellulase production was recorded with 3% CMC and 1% lactose when added individually in the Omeliansky’s medium. The optimum temperature and time for cellulase production by this bacterial strain was 37°C and 10 days, respectively. To our knowledge this is the first report on enhancement of cellulase production by lactose in the Microbacterium sp.