MLVA and Phage Typing as Complementary Tools in the Epidemiological Investigation of Salmonella enterica serovar Typhimurium Clusters

In South Australia serotyping and phage typing are employed for routine Salmonella surveillance. Molecular techniques such as Multiple-locus variable number tandem repeat analysis (MLVA) are increasingly utilized to aid outbreak investigations. During 2007 three Salmonella enterica serovar Typhimuri...

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Bibliographic Details
Published in:Current microbiology 2011-03, Vol.62 (3), p.1034-1038
Main Authors: Ross, Ian L, Davos, Dianne E, Mwanri, Lillian, Raupach, Jane, Heuzenroeder, Michael W
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Bacteria
Bacteriophage Typing
Biomedical and Life Sciences
Biotechnology
Child
Child, Preschool
DNA, Bacterial - genetics
Environmental Microbiology
Epidemiologic Methods
Epidemiology
Female
Food Microbiology
Geography
Humans
Infant
Life Sciences
Male
Microbiology
Middle Aged
Minisatellite Repeats
Molecular Typing
Outbreaks
Salmonella
Salmonella enterica
Salmonella Infections - epidemiology
Salmonella Infections - microbiology
Salmonella typhimurium - classification
Salmonella typhimurium - isolation & purification
Serotyping
South Australia - epidemiology
Time Factors
Young Adult
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Summary:In South Australia serotyping and phage typing are employed for routine Salmonella surveillance. Molecular techniques such as Multiple-locus variable number tandem repeat analysis (MLVA) are increasingly utilized to aid outbreak investigations. During 2007 three Salmonella enterica serovar Typhimurium outbreaks involving phage types DT9, DT29, and DT44 were investigated. Human, food and environmental isolates were also typed by MLVA. In the DT9 outbreak cluster MLVA demonstrated distinct groupings that corresponded to epidemiological differences in time, place, and descriptive information on potential transmission mechanisms. In contrast, the human and food isolates of both the DT29 and DT44 clusters had identical MLVA profiles for all but one case. These data correlated with the epidemiology suggesting that these isolates were closely related and probably a single agent. These findings illustrate that phage typing and MLVA can provide different but complementary information for epidemiological investigations of Salmonella outbreaks.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-010-9820-1