Aerobic and anaerobic regulation of the ubiCA operon, encoding enzymes for the first two committed steps of ubiquinone biosynthesis in Escherichia coli

The ubiCA operon of Escherichia coli encodes enzymes for the first two steps of ubiquinone biosynthesis. A monolysogen ( ubiC-lacZ operon fusion) was constructed to study ubiCA regulation. Expression was higher during aerobic growth than anaerobically, and increased with rate of oxygen supply. Altho...

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Bibliographic Details
Published in:FEBS letters 1997-09, Vol.414 (2), p.373-376
Main Authors: Søballe, Britta, Poole, Robert K.
Format: Article
Language:English
Subjects:
Aerobiosis
Alkyl and Aryl Transferases
Anaerobiosis
Base Sequence
beta-Galactosidase - biosynthesis
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fnr
Gene expression ( E. coli)
Gene Expression Regulation, Bacterial - drug effects
Gene Expression Regulation, Enzymologic - drug effects
Genotype
Glucose - pharmacology
Hydrogen Peroxide - pharmacology
IHF
Kinetics
Molecular Sequence Data
Operon
Oxo-Acid-Lyases - biosynthesis
Oxo-Acid-Lyases - genetics
Paraquat - pharmacology
Promoter Regions, Genetic
Recombinant Fusion Proteins - biosynthesis
Transferases - biosynthesis
Transferases - genetics
Ubi genes
Ubiquinone - biosynthesis
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Summary:The ubiCA operon of Escherichia coli encodes enzymes for the first two steps of ubiquinone biosynthesis. A monolysogen ( ubiC-lacZ operon fusion) was constructed to study ubiCA regulation. Expression was higher during aerobic growth than anaerobically, and increased with rate of oxygen supply. Although ubiquinone is implicated in antioxidant roles, ubiC expression was not elevated in response to hydrogen peroxide or the redox cycling agent, paraquat. Glucose repressed expression and mutation of cya (encoding adenylate cyclase) increased expression. Anaerobically utilised electron acceptors (nitrite, nitrate, fumarate) did not affect expression. ubiC expression appears to be negatively regulated by Fnr and IHF.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)01041-7