Establishment of a novel chinese human lung adenocarcinoma cell line CPA-Yang3 and its real bone metastasis clone CPA-Yang3BM in immunodeficient mice

• Published in: Zhongguo fei ai za zhi 2011-02, Vol.14 (2), p.79-85
• Main Authors: Yang, Shunfang, Shi, Meiping, Cao, Jie, Su, Jianzhong, Zhao, Lanxiang, Lei, Bei, Chang, Cheng, Lu, Jianying, Ye, Jianding, Xie, Wenhui
• Format: Article
• Language: Chinese
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2011.02.01

BACKGROUND AND OBJECTIVEThe recurrence and metastasis of lung cancer is a tough problem worldwide. The aim of this study is to establish a novel Chinese lung adenocarcinoma cell line and its real bone-seeking clone sub-line for exploring the molecular mechanism of lung cancer metastasis.METHODSThe cells came from the pleural effusion of a sixty-five years old female patient with lung adenocarcinoma and supraclavicular lymph node metastases. The gene expression was detected by real-time quantitative PCR. Intracardiac injection of the cells into nude mice was performed and in vivo imaging was obtained by bone scintigraphy and conventional radiography. Bone metastases were determined on bone scintigraphy and then the lesions were resected under deep anesthesia for bone metastasis cancer cell culture. The process was repeated for four cycles to obtain a real bone-seeking clone.RESULTSThe tumorigenesis rate started at 4th passage in immunodeficient mice via subcutaneously and as well as later passages. Approximate