Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method

Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2011-03, Vol.49 (3), p.1137-1139
Main Authors: Moure, Raquel, Muñoz, Laura, Torres, Miriam, Santin, Miguel, Martín, Rogelio, Alcaide, Fernando
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
DNA-Directed RNA Polymerases
Drug Resistance, Bacterial
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Mutant Proteins - genetics
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Time Factors
Tuberculosis - diagnosis
Tuberculosis - microbiology
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Summary:Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01831-10