Mechanism of Regulation of Na-H Exchanger in Inflammatory Bowel Disease: Role of TLR-4 Signaling Mechanism

• Published in: Digestive diseases and sciences 2011-06, Vol.56 (6), p.1656-1662
• Main Authors: Siddique, Iqbal, Khan, Islam
• Format: Article
• Language: English
• Subjects: Adult; Aminosalicylic acids; Analysis; Azathioprine; Biochemistry; Biological and medical sciences; Care and treatment; Cellular signal transduction; Colitis, Ulcerative - genetics; Colitis, Ulcerative - metabolism; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation - physiology; Hepatology; Humans; Inflammatory bowel disease; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Muscle proteins; Myeloid Differentiation Factor 88 - genetics; Myeloid Differentiation Factor 88 - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; Oncology; Original Article; Other diseases. Semiology; Signal Transduction - physiology; Sodium-Hydrogen Exchangers - genetics; Sodium-Hydrogen Exchangers - metabolism; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Transplant Surgery; Ulcerative colitis; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-010-1524-7

Objective We investigated the role of toll-like receptor-4 (TLR-4) signal transduction in the regulation of Na-H exchanger-1 isoform (NHE-1) in ulcerative colitis (UC). Methods Colonic biopsies from control and UC patients were selected from four groups: controls (group 1), untreated UC patients (group 2), UC patients treated with 5′-aminosalicylic acid (5′-ASA) plus steroid (group 3), and UC patients treated with 5′-ASA plus azathioprine (AZA) (group 4). Patients presenting with abdominal pain ( n  = 13) and a normal colon on endoscopy served as controls. NHE-1, TLR-4, MyD88, NFkB and actin protein levels were estimated using Western blot analysis and sodium pump activity (PNPase) by a spectrophotometeric method. Myeloperoxidase (MPO) activity and histologic evaluation confirmed inflammation. Results PNPase activity decreased significantly ( P