Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae)

• Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure. • Methods and Results: Six polymorphic and six monomorphic microsatell...

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Bibliographic Details
Published in:American journal of botany 2011-06, Vol.98 (6), p.e155-e157
Main Authors: Jiang, Kai, Shi, Yi‐Su, Zhang, Jian, Xu, Na‐Na
Format: Article
Language:English
Subjects:
Alleles
clonal growth
DNA Primers - genetics
Endangered Species
Gene loci
Genetic Loci - genetics
Genetic markers
Genetics, Population
Halophila beccarii
Heterozygote
Hydrocharitaceae
Hydrocharitaceae - genetics
Marine
microsatellite primer
Microsatellite Repeats - genetics
Molecular Sequence Data
Plant populations
Plant reproduction
Polymorphism
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Summary:• Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure. • Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737. • Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100032