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Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae)
• Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure. • Methods and Results: Six polymorphic and six monomorphic microsatell...
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| Published in: | American journal of botany 2011-06, Vol.98 (6), p.e155-e157 |
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| container_end_page | e157 |
| container_issue | 6 |
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| container_title | American journal of botany |
| container_volume | 98 |
| creator | Jiang, Kai Shi, Yi‐Su Zhang, Jian Xu, Na‐Na |
| description | • Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure.
• Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737.
• Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale. |
| doi_str_mv | 10.3732/ajb.1100032 |
| format | article |
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• Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737.
• Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale.</description><identifier>ISSN: 0002-9122</identifier><identifier>EISSN: 1537-2197</identifier><identifier>DOI: 10.3732/ajb.1100032</identifier><identifier>PMID: 21613064</identifier><identifier>CODEN: AJBOAA</identifier><language>eng</language><publisher>United States: Botanical Society of America</publisher><subject>Alleles ; clonal growth ; DNA Primers - genetics ; Endangered Species ; Gene loci ; Genetic Loci - genetics ; Genetic markers ; Genetics, Population ; Halophila beccarii ; Heterozygote ; Hydrocharitaceae ; Hydrocharitaceae - genetics ; Marine ; microsatellite primer ; Microsatellite Repeats - genetics ; Molecular Sequence Data ; Plant populations ; Plant reproduction ; Polymorphism</subject><ispartof>American journal of botany, 2011-06, Vol.98 (6), p.e155-e157</ispartof><rights>2011 Botanical Society of America</rights><rights>Copyright Botanical Society of America, Inc. Jun 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3895-6a3b7eda3df4636133a44b1a8e4621bb5d7464590e2aec63dc86ca22bb87098a3</citedby><cites>FETCH-LOGICAL-c3895-6a3b7eda3df4636133a44b1a8e4621bb5d7464590e2aec63dc86ca22bb87098a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21613064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Kai</creatorcontrib><creatorcontrib>Shi, Yi‐Su</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Xu, Na‐Na</creatorcontrib><title>Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae)</title><title>American journal of botany</title><addtitle>Am J Bot</addtitle><description>• Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure.
• Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737.
• Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale.</description><subject>Alleles</subject><subject>clonal growth</subject><subject>DNA Primers - genetics</subject><subject>Endangered Species</subject><subject>Gene loci</subject><subject>Genetic Loci - genetics</subject><subject>Genetic markers</subject><subject>Genetics, Population</subject><subject>Halophila beccarii</subject><subject>Heterozygote</subject><subject>Hydrocharitaceae</subject><subject>Hydrocharitaceae - genetics</subject><subject>Marine</subject><subject>microsatellite primer</subject><subject>Microsatellite Repeats - genetics</subject><subject>Molecular Sequence Data</subject><subject>Plant populations</subject><subject>Plant reproduction</subject><subject>Polymorphism</subject><issn>0002-9122</issn><issn>1537-2197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkb1PwzAQxS0EouVjYkcRCyCU4q_YzggVUFARS5mjs3OlqdKm2A2o_z2uWhgYYDq9009Pd-8RcsJoT2jBr2Fqe4xRSgXfIV2WCZ1ylutd0o07nuaM8w45CGEaZS5zvk86nCkmqJJdMnqunG8CLLGuqyUmC1_N0Idk3Pjko63n6MHWmASENw8hJAOom8WkqiGx6Bz4qkouBqvSN24SxRIcAl4ekb0x1AGPt_OQvN7fjfqDdPjy8Ni_GaZOmDxLFQirsQRRjqUS8SIBUloGBqXizNqs1FLJLKfIAZ0SpTPKAefWGk1zA-KQnG98F755bzEsi1kVXPwE5ti0ocipZkpnMvuXNJoJY4QUkTz7RU6b1s_jGxGKuVLD1tDVBlpnFzyOi3Vu4FcFo8W6lCKWUmxLifTp1rK1Myx_2O8WIsA2wGdV4-ovr-Lm6ZbfsSwTX595lbM</recordid><startdate>201106</startdate><enddate>201106</enddate><creator>Jiang, Kai</creator><creator>Shi, Yi‐Su</creator><creator>Zhang, Jian</creator><creator>Xu, Na‐Na</creator><general>Botanical Society of America</general><general>Botanical Society of America, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201106</creationdate><title>Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae)</title><author>Jiang, Kai ; Shi, Yi‐Su ; Zhang, Jian ; Xu, Na‐Na</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3895-6a3b7eda3df4636133a44b1a8e4621bb5d7464590e2aec63dc86ca22bb87098a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alleles</topic><topic>clonal growth</topic><topic>DNA Primers - genetics</topic><topic>Endangered Species</topic><topic>Gene loci</topic><topic>Genetic Loci - genetics</topic><topic>Genetic markers</topic><topic>Genetics, Population</topic><topic>Halophila beccarii</topic><topic>Heterozygote</topic><topic>Hydrocharitaceae</topic><topic>Hydrocharitaceae - genetics</topic><topic>Marine</topic><topic>microsatellite primer</topic><topic>Microsatellite Repeats - genetics</topic><topic>Molecular Sequence Data</topic><topic>Plant populations</topic><topic>Plant reproduction</topic><topic>Polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Kai</creatorcontrib><creatorcontrib>Shi, Yi‐Su</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Xu, Na‐Na</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>American journal of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Kai</au><au>Shi, Yi‐Su</au><au>Zhang, Jian</au><au>Xu, Na‐Na</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae)</atitle><jtitle>American journal of botany</jtitle><addtitle>Am J Bot</addtitle><date>2011-06</date><risdate>2011</risdate><volume>98</volume><issue>6</issue><spage>e155</spage><epage>e157</epage><pages>e155-e157</pages><issn>0002-9122</issn><eissn>1537-2197</eissn><coden>AJBOAA</coden><abstract>• Premise of the study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure.
• Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737.
• Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale.</abstract><cop>United States</cop><pub>Botanical Society of America</pub><pmid>21613064</pmid><doi>10.3732/ajb.1100032</doi><tpages>3</tpages></addata></record> |
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| ispartof | American journal of botany, 2011-06, Vol.98 (6), p.e155-e157 |
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| language | eng |
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| source | JSTOR-E-Journals; Wiley-Blackwell Read & Publish Collection; Botanical Society of America |
| subjects | Alleles clonal growth DNA Primers - genetics Endangered Species Gene loci Genetic Loci - genetics Genetic markers Genetics, Population Halophila beccarii Heterozygote Hydrocharitaceae Hydrocharitaceae - genetics Marine microsatellite primer Microsatellite Repeats - genetics Molecular Sequence Data Plant populations Plant reproduction Polymorphism |
| title | Microsatellite primers for vulnerable seagrass Halophila beccarii (Hydrocharitaceae) |
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