p53 mutation and loss have different effects on tumourigenesis in a novel mouse model of pleomorphic rhabdomyosarcoma

• Published in: The Journal of pathology 2010-10, Vol.222 (2), p.129-137
• Main Authors: Doyle, Brendan, Morton, Jennifer P, Delaney, David W, Ridgway, Rachel A, Wilkins, Julie A, Sansom, Owen J
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Biological and medical sciences; Cell Transformation, Neoplastic - genetics; Chromosomal Instability; Data processing; Development; Disease Models, Animal; Diseases of the osteoarticular system; Genes, p53 - genetics; Investigative techniques, diagnostic techniques (general aspects); K-Ras protein; Life span; Loss of Heterozygosity; Medical sciences; Metastases; Mice; Mice, Transgenic; muscle; Muscle, Skeletal - metabolism; p53; p53 protein; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; pleomorphic rhabdomyosarcoma; Point Mutation; Proto-Oncogene Proteins p21(ras) - genetics; Rhabdomyosarcoma; Rhabdomyosarcoma - genetics; Rhabdomyosarcoma - metabolism; Rhabdomyosarcoma - secondary; Soft Tissue Neoplasms - genetics; Soft Tissue Neoplasms - metabolism; transgenic mouse model; Tumor Suppressor Protein p53 - metabolism; Tumors of striated muscle and skeleton
• ISSN: 0022-3417; 1096-9896
• DOI: 10.1002/path.2748

Pleomorphic rhabdomyosarcoma is the most common variant of this tumour in adults and has a very poor outcome. Two genes which are known to play a role in rhabdomyosarcoma development are KRas and p53. In the majority of human tumours, p53 abnormalities are point mutations that result in the expression of a mutant form of the protein. It is now hypothesized that these mutant forms of p53 may be playing an oncogenic role, over and above simple loss of the wild-type function. In this study, we use Cre-LoxP technology to develop a novel mouse model of rhabdomyosarcoma, crossing mice expressing a common KRas mutation (G12V) with mice that either lose p53 expression or express a mutant form of p53. We use this model to explore the different effects of p53 loss and mutation in the setting of an activating KRas mutation. We found that either complete loss of p53 (p53$^{\rm{fl/fl}}$) or the expression of one mutant p53 allele with concomitant loss of the second allele (p53$^{\rm{R172H/fl}}$) resulted in the rapid development of rhabdomyosarcoma in 15/16 and 19/19 mice, respectively. In contrast, there was a marked difference between mice which lose a single copy of p53 (p53$^{{\rm{fl}}/+}$) and mice expressing a single copy of mutant p53 (p53$^{{\rm {172H}}/+}$). Fourteen out of 16 p53$^{{\rm{R172H}}/+}$ mice developed rhabdomyosarcoma, compared with two out of 31 p53$^{{\rm{fl}}/+}$ mice. As a consequence of this, p53$^{{\rm{fl}}/+}$ mice had a median lifespan nearly double that of the p53$^{{\rm{R172H}}/+}$ mice. To underline the enhanced effect of p53 mutation in tumour progression, metastases were seen only in those mice which expressed the mutant form. These data demonstrate that mutant p53 can co-operate with activated, mutant KRas to influence tumourigenesis and metastatic potential, over and above simple loss of normal protein function. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.