Acute Restraint Stress in Zebrafish: Behavioral Parameters and Purinergic Signaling

Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular para...

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Bibliographic Details
Published in:Neurochemical research 2011-10, Vol.36 (10), p.1876-1886
Main Authors: Piato, Angelo L., Rosemberg, Denis B., Capiotti, Katiucia M., Siebel, Anna M., Herrmann, Ana P., Ghisleni, Gabriele, Vianna, Monica R., Bogo, Maurício R., Lara, Diogo R., Bonan, Carla D.
Format: Article
Language:English
Subjects:
Adenosine Deaminase - genetics
Adenosine Deaminase - metabolism
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Animals
Behavior, Animal - physiology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Danio rerio
Freshwater
Male
Memory - physiology
Motor Activity - physiology
Neurochemistry
Neurology
Neurosciences
Original Paper
Purines - metabolism
Restraint, Physical
Signal Transduction - physiology
Stress, Psychological
Zebrafish - physiology
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular parameters in zebrafish brain. Animals were submitted to a 90 min ARS protocol and tested for anxiety levels, exploratory behavior, and memory performance. Furthermore, we analyzed ectonucleotidase and adenosine deaminase activities and their gene expression profile, as well as transcription of adenosine receptors. ARS increased anxiety, but did not impair locomotion or cognition. ARS significantly increased ATP hydrolysis, decreased cytosolic ADA activity, and changed the entpd and adora gene expression. In conclusion, ARS disturbed zebrafish behavior, and we hypothesize that the augmentation in adenosine-mediated signaling may be a strategy to reestablish homeostasis and normal behavior after a stressful event.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-011-0509-z