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Light-induced noncovalent fixation of DNA and synthetic polyions on the surface of silicon single crystals

The paper presents a simple method for fixing DNA and synthetic polyions on the surface of p and n type Si(100) single crystals. Different methods for preparing the surface for DNA fixation are compared. It is shown that the conformation of macromolecules fixed on a substrate depends on the presence...

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Bibliographic Details
Published in:Journal of structural chemistry 2009-10, Vol.50 (5), p.962-969
Main Authors: Volkov, I. L., Bazlov, N. V., Bondarenko, A. S., Vyvenko, O. F., Kas’yanenko, N. A.
Format: Article
Language:English
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Summary:The paper presents a simple method for fixing DNA and synthetic polyions on the surface of p and n type Si(100) single crystals. Different methods for preparing the surface for DNA fixation are compared. It is shown that the conformation of macromolecules fixed on a substrate depends on the presence of bivalent counterions in solution and the charge properties of the surface and polymer. The results of fixation were checked and analyzed by atomic force microscopy (AFM).
ISSN:0022-4766
1573-8779
DOI:10.1007/s10947-009-0143-7