Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles

We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-09, Vol.27 (1), p.77-81
Main Authors: Verdoold, Remco, Gill, Ron, Ungureanu, Felicia, Molenaar, Robert, Kooyman, Rob P.H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Colorimetry - instrumentation
Colorimetry - methods
Darkfield microscopy
DNA - analysis
DNA - chemistry
DNA sensing
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Gold nanoparticles
Local surface plasmon resonance
Metal Nanoparticles - chemistry
Methods. Procedures. Technologies
Microscopy - instrumentation
Microscopy - methods
Nucleic Acid Hybridization
Optical detection
Various methods and equipments
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Summary:We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0 × 10 −14 M without the need for a spectrometer or a scanning stage.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2011.06.019