A novel method for the extraction and culture of progenitor stem cells from human peripheral blood for use in regenerative medicine

Human peripheral blood (HPB) contains both circulating endothelial cells (CECs) and endothelial progenitor stem cells (EPCs), which may be suitable for use in regenerative medicine. There has been considerable interest in using these cells, but there is no “gold standard” technique for isolating the...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2011-09, Vol.58 (5), p.328-334
Main Authors: Punshon, Geoffrey, Vara, Dina S., Sales, Kevin M., Seifalian, Alexander M.
Format: Article
Language:English
Subjects:
Adult
Animals
cardiovascular implants
Cattle
Cell Separation - methods
Cells, Cultured
Coated Materials, Biocompatible - metabolism
Endothelial Cells - cytology
Endothelial Cells - metabolism
endothelial progenitor cell
Fibronectins - metabolism
Gelatin - metabolism
Humans
peripheral blood
Regenerative Medicine
RNA - analysis
Serum - metabolism
Stem Cells - cytology
Stem Cells - metabolism
tissue engineering
Tissue Engineering - methods
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Summary:Human peripheral blood (HPB) contains both circulating endothelial cells (CECs) and endothelial progenitor stem cells (EPCs), which may be suitable for use in regenerative medicine. There has been considerable interest in using these cells, but there is no “gold standard” technique for isolating these cells. The aim of this study was to characterize and compare a number of different extraction and culture techniques to develop a system to isolate and culture cells. EPC and CEC were isolated from HPB using either Histopaque‐1077 or Lymphoprep. The two isolation methods were compared for the number of cells isolated, cell metabolism, and RNA expression. Both isolations produced viable cells and were comparable. The tissue culture method employed does have a significant effect on the cell population with regard to medium choice, fetal bovine serum concentration, and surface modification of the culture surface. In conclusion, it can be seen that although this study and previous work can suggest a basis for culture, further work to develop an optimized and agreed “gold standard” culture regime for EPC from HPB is required to maximize the potential of this source of cells for regenerative medicine and to translate its clinical use in the future.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.47