Conserved peptide sequences bind to actin and enolase on the surface of Plasmodium berghei ookinetes

The description of Plasmodium ookinete surface proteins and their participation in the complex process of mosquito midgut invasion is still incomplete. In this study, using phage display, a consensus peptide sequence (PWWP) was identified in phages that bound to the Plasmodium berghei ookinete surfa...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology 2011-09, Vol.138 (11), p.1341-1353
Main Authors: HERNÁNDEZ-ROMANO, J., RODRÍGUEZ, M. H., PANDO, V., TORRES-MONZÓN, J. A., ALVARADO-DELGADO, A., LECONA VALERA, A. N., RAMOS, R. ARGOTTE, MARTÍNEZ-BARNETCHE, J., RODRÍGUEZ, M. C.
Format: Article
Language:English
Subjects:
Actins - metabolism
Aedes - genetics
Aedes - metabolism
Aedes - parasitology
Amino Acid Sequence
Animals
Anopheles - genetics
Anopheles - metabolism
Anopheles - parasitology
Aquatic insects
Biological and medical sciences
Cell Extracts
Cells, Cultured
Conserved Sequence
Digestive System - metabolism
Digestive System - parasitology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Invertebrates
Life Cycle Stages - genetics
Malaria - metabolism
Malaria - parasitology
Male
Mice
Mice, Inbred BALB C
Microscopy, Electron
Molecular Sequence Data
Mosquitoes
Peptide Library
Peptides - chemistry
Peptides - genetics
Peptides - metabolism
Phosphopyruvate Hydratase - metabolism
Plasmodium berghei
Plasmodium berghei - genetics
Plasmodium berghei - metabolism
Protozoan Proteins - metabolism
Rodent Diseases - metabolism
Rodent Diseases - parasitology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The description of Plasmodium ookinete surface proteins and their participation in the complex process of mosquito midgut invasion is still incomplete. In this study, using phage display, a consensus peptide sequence (PWWP) was identified in phages that bound to the Plasmodium berghei ookinete surface and, in selected phages, bound to actin and enolase in overlay assays with ookinete protein extracts. Actin was localized on the surface of fresh live ookinetes by immunofluorescence and electron microscopy using specific antibodies. The overall results indicated that enolase and actin can be located on the surface of ookinetes, and suggest that they could participate in Plasmodium invasion of the mosquito midgut.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182011001296