Short-Term Storage of Human Spermatozoa in Electrolyte-Free Medium Without Freezing Maintains Sperm Chromatin Integrity Better Than Cryopreservation

Previous attempts to maintain human spermatozoa without freezing were based on short-term storage in component-rich medium and led to fast decline in motility and increased incidence of chromosome breaks. Here we report a new method in which sperm are maintained without freezing in an electrolyte-fr...

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Bibliographic Details
Published in:Biology of reproduction 2011-09, Vol.85 (3), p.536-547
Main Authors: RIEL, Jonathan M, YAMAUCHI, Yasuhiro, HUANG, Thomas T. F, GROVE, John, WARD, Monika A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Survival
Chromatin - chemistry
Cryopreservation
Culture Media - chemistry
DNA Fragmentation
Female
Fertilization
Fundamental and applied biological sciences. Psychology
Glucose
Humans
Male
Mice
Semen Preservation - methods
Serum Albumin, Bovine
Sperm Count
Sperm Injections, Intracytoplasmic
Sperm Motility
Vertebrates: reproduction
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Summary:Previous attempts to maintain human spermatozoa without freezing were based on short-term storage in component-rich medium and led to fast decline in motility and increased incidence of chromosome breaks. Here we report a new method in which sperm are maintained without freezing in an electrolyte-free medium (EFM) composed of glucose and bovine serum albumin. Human sperm were stored in EFM or human tubal fluid medium (HTFM) or were cryopreserved, and their motility, viability, and DNA integrity were examined at different intervals. Cryopreservation led to significant decline in sperm motility and viability and induced DNA fragmentation. Sperm stored in EFM maintained motility and viability for up to 4 and 7 wk, respectively, much longer than sperm stored in HTFM (
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.111.091322