Neurochemical and electrophysiological changes induced by paradoxical sleep deprivation in rats

• Published in: Behavioural brain research 2011-11, Vol.225 (1), p.39-46
• Main Authors: Mohammed, Haitham S., Aboul Ezz, Heba S., Khadrawy, Yasser A., Noor, Neveen A.
• Format: Article
• Language: English
• Subjects: Amino acid neurotransmitters; Amino Acids - metabolism; Animals; Behavioral psychophysiology; Biological and medical sciences; Brain Waves - physiology; Cerebral Cortex - metabolism; Cerebral Cortex - physiopathology; Cortex; Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes; EEG; Electrodes, Implanted; Electroencephalography - methods; Fundamental and applied biological sciences. Psychology; Hippocampus; Hippocampus - metabolism; Hippocampus - physiopathology; Male; Medical sciences; Nervous system (semeiology, syndromes); Neurochemistry; Neurology; Paradoxical sleep deprivation; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rat; Rats; Rats, Wistar; Sleep Deprivation - metabolism; Sleep Deprivation - pathology; Sleep Deprivation - physiopathology; Spectrum Analysis; Weight Gain - physiology
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2011.06.018

► The study of the effects of PSD on the waking EEG and amino acid neurotransmitters in rats revealed changes from normal animals. ► After 24 h delta power increased and the rest of EEG waves decreased. While, after 48 h and 72 h the waves showed slight changes from control. ► Increase in cortical glutamate, glycine and taurine and in hippocampal glutamate, aspartate, glutamine and glycine has been detected. ► Waking EEG and neurotransmitter analyses suggest that PSD induced neurochemical and electrophysiological changes in the rat's brain. The present study aims to investigate the effects of paradoxical sleep deprivation (PSD) on the waking EEG and amino acid neurotransmitters in the hippocampus and cortex of rats. Animals were deprived of paradoxical sleep for 72 h by using the multiple platform method. The EEG power spectral analysis was carried out to assess the brain's electrophysiological changes due to sleep deprivation. The concentrations of amino acid neurotransmitters were assessed in the hippocampus and cortex using HPLC. Control data showed slight differences from normal animals in the delta, theta and alpha waves while an increase in the beta wave was obtained. After 24 h of PSD, delta relative power increased and the rest of EEG wave's power decreased with respect to control. After 48 h and 72 h the spectral power analysis showed non-significant changes to control. The amino acid neurotransmitter analysis revealed a significant increase in cortical glutamate, glycine and taurine levels while in the hippocampus, glutamate, aspartate, glutamine and glycine levels increased significantly. Both the waking EEG and neurotransmitter analyses suggest that PSD induced neurochemical and electrophysiological changes that may affect brain proper functionality.