Influence of threonine exporters on threonine production in Escherichia coli

Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine-re...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2002-07, Vol.59 (2-3), p.205-210
Main Authors: KRUSE, D, KRÄMER, R, EGGELING, L, RIEPING, M, PFEFFERLE, W, TCHIEU, J. H, CHUNG, Y. J, SAIER, M. H, BURKOVSKI, A
Format: Article
Language:English
Subjects:
Amino Acid Transport Systems, Neutral
Amino acids
Bacteria
Bacterial Proteins
Biological and medical sciences
Biological Transport
Biotechnology
Carrier Proteins - genetics
Corynebacterium glutamicum
E coli
Enzymes
Escherichia coli - metabolism
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Membrane Proteins - genetics
Metabolites
Methods. Procedures. Technologies
Modification of gene expression level
Threonine - biosynthesis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine-resistant phenotype when overexpressed, and they decrease the accumulation of radioactive metabolites derived from [(14)C] L-threonine. The evidence presented supports the conclusion that both RhtB and RhtC catalyze efflux of L-threonine and other structurally related neutral amino acids, but that the specificities of these two carriers differ substantially.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-002-0987-7