Heat shock protein 60 from Klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone

• Published in: Microbial pathogenesis 2011-11, Vol.51 (5), p.352-359
• Main Authors: Ortega-Ortega, Yolanda, García-Latorre, Ethel Awilda, Lezama, Ruth Angélica, Luna-Herrera, Julieta, Vega-López, Armando, Domínguez-López, María Lilia
• Format: Article
• Language: English
• Subjects: Amiloride; Apoptosis; Apoptosis - drug effects; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; CD4 antigen; CD8 antigen; Cell culture; Cells, Cultured; Chaperonin 60 - genetics; Chaperonin 60 - metabolism; Contractility; cytochalasin D; Cytoskeleton; Dexamethasone; Dexamethasone - toxicity; Escherichia coli; flow cytometry; Fundamental and applied biological sciences. Psychology; Heat shock proteins; Heath shock protein; Hsp60 protein; Humans; Klebsiella Infections - microbiology; Klebsiella Infections - physiopathology; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Leukocytes (mononuclear); Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - microbiology; Lymphocytes T; Microbiology; Miscellaneous; Mononuclear cells; Nocodazole; Peripheral blood mononuclear cells; protective effect; T-lymphocytes
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2011.07.001

Bacterial heat shock proteins can have anti-apoptotic effects on human cells. We investigated whether enterobacterial HSP60 can protect peripheral blood mononuclear cells (PBMC) from DXM-induced apoptosis and if this effect requires cytoskeleton participation. Anti-apoptotic effect from enterobacterial HSP60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before DXM induction. Percentage of apoptotic cells was determined by SubG0 peak and TUNEL techniques in a flow cytometer. Our results showed significant protective effect of HSP60 Klebsiella pneumoniae and E. coli, in the DXM-induced apoptosis in PBMC. Similar results were obtained with recombinant human HSP60. The same protective effect of proteins was observed in CD4+ and CD8 + T cell subpopulations. To analyze if enterobacterial HSP60 need internalization to have the anti-apoptotic effect, we used cytoskeleton inhibitors such as: nocodazole, cytochalasin D and amiloride, the three inhibitors significantly affected the protective role of HSP60 in apoptosis induced with DXM. Results suggest that the protective effect of HSP60 K. pneumoniae and E. coli requires the participation of contractile structures for the internalization of this protein by the cells, we suggest that the internalization of enterobacterial HSP60 could be carry out by macropinocytosis. We report for the first time that K. pneumoniae and E. coli HSP60 have protective effect in the apoptosis induced with DXM in PBMC from healthy subjects and that this effect requires the internalization of the protein with active participation of the cytoskeleton. ► We analyzed for first time the anti-apoptotic effect of enterobacterial HSP60. ► Anti-apoptotic effect of HSP60 was observed in the DXM-induced apoptosis in PBMC. ► The same effect of proteins was observed in CD4+ and CD8+ T cell subpopulations. ► This effect requires internalization of HSP60 with cytoskeleton participation.