Hydroalcoholic extract based-ointment from Punica granatum L. peels with enhanced in vivo healing potential on dermal wounds

• Published in: Phytomedicine (Stuttgart) 2011-08, Vol.18 (11), p.976-984
• Main Authors: Hayouni, E.A., Miled, K., Boubaker, S., Bellasfar, Z., Abedrabba, M., Iwaski, H., Oku, H., Matsui, T., Limam, F., Hamdi, M.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents - pharmacology; Antioxidants - metabolism; Aspergillus niger; Biochemical; Biological activities; Candida albicans; Candida glabrata; Chemical properties; Chromatography, High Pressure Liquid; Escherichia coli; Female; Guinea Pigs; Health aspects; Histopathological; Klebsiella pneumoniae; Male; Materia medica, Vegetable; Microbial Sensitivity Tests - methods; Ointment; Ointments - chemistry; Ointments - pharmacology; Plant extracts; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - pharmacology; Pomegranate; Pseudomonas aeruginosa; Punica granatum; Punica granatum L. peels; Punicaceae - chemistry; Salmonella anatum; Salmonella typhimurium; Skin - drug effects; Skin - injuries; Staphylococcus aureus; Streptococcus pneumoniae; Tensile Strength; Wound healing; Wound Healing - drug effects; Wounds and Injuries - drug therapy
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2011.02.011

The present study reports for the first time, the in vivo wound healing potential of Punica granatum L. peels. A 5% (w/w) methanolic extract based-ointment was formulated and evaluated for its wound healing in guinea pigs. The ointment was applied in vivo on the paravertebral area of twelve excised wounded models once a day for 10 consecutive days. The ointment significantly enhanced the wound contraction and the period of epithelialization as assessed by the mechanical (contraction rate, tensile strength), the biochemical (increasing of collagen, DNA and proteins synthesis) and the histopathological characteristics. Such investigation was encouraged by the efficiency of the methanolic extract as antimicrobial and antioxidant. Indeed, the extract showed antioxidant activity as strong as natural and synthetic compounds (Trolox, BHA, Quercetin). Furthermore, the extract exhibited significant antibacterial and antifungal activity against almost all tested bacteria: Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Salmonella anatum, Salmonella typhimurium, Streptococcus pneumoniae, and fungi Candida albicans, Candida glabrata, Trichopyton rubrum and Aspergillus niger. The formulated ointment might well find use as skin repair agent without hazard to human health based on these results and on the fact that it has been well established that the extracts of pomegranate used in conditions similar to those applied by traditional medicine, showed no toxic effects.