Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species

Abstract Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2011-11, Vol.71 (3), p.304-308
Main Authors: Santos, Cledir, Lima, Nelson, Sampaio, Paula, Pais, Célia
Format: Article
Language:English
Subjects:
Biological and medical sciences
C. parapsilosis complex
Candida
Candida - classification
Candida - isolation & purification
Candidiasis - diagnosis
Fundamental and applied biological sciences. Psychology
Humans
Infectious Disease
Infectious diseases
Internal Medicine
MALDI-TOF ICMS
Medical sciences
Microbiology
Miscellaneous
Mycology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Abstract Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis , and C. metapsilsosis , and very closely related species like C. glabrata and C. bracarensis , and C. albicans and C. dubliniensis could be clearly separated. MALDI-TOF-ICMS stands out as a promising tool for the rapid detection of emerging pathogens.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2011.07.002