Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls

Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspension...

Full description

Saved in:
Bibliographic Details
Published in:Journal of fluorescence 2010, Vol.20 (1), p.343-352
Main Authors: Plasek, Jaromir, Hoskova, Barbora
Format: Article
Language:English
Subjects:
Absorption
Analytical Chemistry
Benzenesulfonates - chemistry
Benzenesulfonates - metabolism
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Cell Wall - metabolism
Chemical Phenomena
Chitin - metabolism
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Hydrogen-Ion Concentration
Original Paper
Saccharomyces cerevisiae - cytology
Solutions
Spectrometry, Fluorescence
Staining and Labeling
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-009-0563-y