Continuous monitoring of hypothalamic neurotransmitters and thermoregulatory responses in exercising rats

• Published in: Journal of neuroscience methods 2011-11, Vol.202 (2), p.119-123
• Main Authors: Hasegawa, Hiroshi, Takatsu, Satomi, Ishiwata, Takayuki, Tanaka, Hideto, Sarre, Sophie, Meeusen, Romain
• Format: Article
• Language: English
• Subjects: Animals; Body Temperature Regulation - physiology; Exercise; Hypothalamus - metabolism; Hypothalamus - secretion; Male; Microdialysis; Monitoring, Physiologic - instrumentation; Monitoring, Physiologic - methods; Neurotransmission; Neurotransmitter Agents - metabolism; Neurotransmitter Agents - secretion; Physical Conditioning, Animal - physiology; Rats; Rats, Wistar; Thermoregulation
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/j.jneumeth.2011.05.024

► Thermoregulatory responses are dependent on the intensity of the exercise. ► Incremental treadmill running increased catecholamine release in the hypothalamus. ► Extracellular serotonin in the hypothalamus was not changed during running. ► Our exercise model is useful to examine thermoregulation and neurotransmission. The purpose of the present study was to investigate the relationship between thermoregulation and catecholamine release in the preoptic area and anterior hypothalamus (PO/AH) during incremental treadmill running in the rat. To this aim, we combined in vivo brain microdialysis, biotelemetry and metabolic measurements for continuous monitoring of core body temperature ( T core), neurotransmitters and thermoregulatory responses. The animals were exercised for 1 h at 23 °C. Treadmill speed was increased every 20 min (10, 20 and 26 m min −1). T core, oxygen consumption ( V ˙ O 2 , an index of heat production) and tail skin temperature ( T tail, an index of heat loss) were simultaneously measured. Brain microdialysis samples were collected every 10 min, and these samples were analyzed for noradrenaline (NA), dopamine (DA) and serotonin (5-HT). T core and V ˙ O 2 significantly increased during treadmill and were exercise intensity dependent. After an initial drop T tail increased significantly during exercise. Both NA and DA levels in the PO/AH increased significantly during exercise. There was no effect on serotonin release. T core, V ˙ O 2 and T tail were positively correlated with the levels of NA and DA. Our data suggest that thermoregulatory responses are dependent on the intensity of the exercise and that these responses are associated with changes in NA and DA release, but not in 5-HT release in the PO/AH.