RNA-cleaving 10–23 deoxyribozyme with a single amino acid-like functionality operates without metal ion cofactors

A series of 10-23 deoxyribozymes (D2-D9) containing single amino-acid-bearing nucleosides (thr6dA, hisam6dA, hisam5dU and ncmnm5dU) at positions 4, 5, 8 or 15 of the catalytic core was obtained by chemical synthesis. The deoxyribozymes were screened for their catalytic efficiency, and in the presenc...

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Bibliographic Details
Published in:New journal of chemistry 2010, Vol.34 (5), p.934-948
Main Authors: Smuga, Damian, Majchrzak, Kinga, Sochacka, Elzbieta, Nawrot, Barbara
Format: Article
Language:English
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Summary:A series of 10-23 deoxyribozymes (D2-D9) containing single amino-acid-bearing nucleosides (thr6dA, hisam6dA, hisam5dU and ncmnm5dU) at positions 4, 5, 8 or 15 of the catalytic core was obtained by chemical synthesis. The deoxyribozymes were screened for their catalytic efficiency, and in the presence of 1 mM Mg2+ two of them, containing at position 8 either hisam5dU (D8) or ncmnm5dU (D9), were found to be RNA nucleases several times more active than their non-modified precursor. Moreover, in the magnesium-free TRIS or PIPES buffers, these enzymes were able to catalyze the cleavage of the phosphodiester bond located between the 5[prime or minute]-GpU-3[prime or minute] sequence of the complementary RNA substrate. The cleavage reaction proceeded with the highest efficiency at pH 7.
ISSN:1144-0546
1369-9261
DOI:10.1039/b9nj00705a