Wild and farm breeding cervids infections with Anaplasma phagocytophilum

The main goal of our study was to determine the prevalence of Anaplasma phagocytophilum infections in wild cervids living in north-eastern part of Poland. Material used in the study was gathered between the years 2004- 2008. The blood samples from 106 red deer (Cervus elaphus), 32 sika deer (Cervus...

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Bibliographic Details
Published in:Annals of Agricultural and Environmental Medicine 2011, Vol.18 (1), p.73-77
Main Authors: Hapunik, Joanna, Víchová, Bronislava, Karbowiak, Grzegorz, Wita, Irena, Bogdaszewski, Marek, Pet'ko, Branislav
Format: Article
Language:English
Subjects:
Anaplasma phagocytophilum
Animals
Animals, Wild
Biotechnology
Blood
Breeding
Capreolus capreolus
Cervidae
Cervus elaphus
Cervus nippon
Cervus nippon hortulorum
Dama dama
Deer
Ehrlichiosis - epidemiology
Ehrlichiosis - veterinary
Female
Male
Poland - epidemiology
Prevalence
rRNA 16S
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Summary:The main goal of our study was to determine the prevalence of Anaplasma phagocytophilum infections in wild cervids living in north-eastern part of Poland. Material used in the study was gathered between the years 2004- 2008. The blood samples from 106 red deer (Cervus elaphus), 32 sika deer (Cervus nippon hortulorum), 130 fallow deer (Dama dama) and 31 roe deer (Capreolus capreolus) were collected. DNA was isolated using Genomic Mini AX blood kit (A and A Biotechnology). Molecular detection of A. phagocytophilum was based on nested PCR amplification of a species-specific 16S rRNA fragment gene of A. phagocytophilum. The highest prevalence of infection was detected in Cervus elaphus, Capreolus capreolus, Cervus nippon hortulorum, there were 50.9 percent, 38.7 percent, 34.37 percent of infected animals, respectively. The lowest rate of infection was found in fallow deer (Dama dama) - ony 1.5 percent.
ISSN:1232-1966
1898-2263