Diazirine-containing RNA photocrosslinking probes for the study of siRNA-protein interactions

We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3'-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally de...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2010-01, Vol.46 (39), p.7367-7369
Main Authors: Kuboe, Satoru, Yoda, Mayuko, Ogata, Aya, Kitade, Yukio, Tomari, Yukihide, Ueno, Yoshihito
Format: Article
Language:English
Subjects:
Azirines - chemistry
Base Sequence
Photochemistry
RNA - chemistry
RNA, Small Interfering
Sequence Homology, Nucleic Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3'-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.
ISSN:1359-7345
1364-548X
DOI:10.1039/c0cc02450c