Type I collagen from bullfrog (Rana catesbeiana) fallopian tube

Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog (Rana catesbeiana) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components ( alpha 1 and alpha 2) and was clas...

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Bibliographic Details
Published in:African journal of biotechnology 2011-08, Vol.10 (42), p.8414-8420
Main Authors: Tzuching, Wang, Shenchung, Lin, Yingpei, Shen, Shangyu, Liu, Tzuling, Wang McCall, Menghsiung, Chin, Tachen, Lin, Clinton, Yang, Winhui, Wu, Chiching, Yang
Format: Article
Language:English
Subjects:
Rana catesbeiana
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Summary:Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog (Rana catesbeiana) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components ( alpha 1 and alpha 2) and was classified as type I collagen. PSC was found to contain 19.5% of amino acids. PSC exhibited a maximum absorbance at 230 nm, but little absorbance near to 280 nm. The denaturation temperature for PSC was determined to be 29.6 degree C. These results suggest that bullfrog fallopian tube has potential as a supplement to the sources of vertebrate collagens.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB10.924