Methotrexate ameliorates pristane-induced arthritis by decreasing IFN- Delta g and IL-17A expressions

Objective This study was carried out to test the effects of methotrexate (MTX) and black seed oil (BSO) on pristane-induced arthritis (PIA) in rats. Methods: Inbred dark agouti (DA) rats were induced by a single subcutaneous injection of pristane, and then treated with MTX or BSO. Arthritis severity...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2011-01, Vol.12 (1), p.40-46
Main Authors: Wei-kun, Hou, Lie-su, Meng, Fang, Zheng, Yu-rong, Wen, Wen-hua, Zhu, Cong-shan, Jiang, Xiao-jing, He, Yan, Zhou, She-min, Lu
Format: Article
Language:English
Subjects:
Arthritis
Cytokines
Methotrexate
Nitric oxide
Rats
Real time
Seeds
Spleen
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Summary:Objective This study was carried out to test the effects of methotrexate (MTX) and black seed oil (BSO) on pristane-induced arthritis (PIA) in rats. Methods: Inbred dark agouti (DA) rats were induced by a single subcutaneous injection of pristane, and then treated with MTX or BSO. Arthritis severity was evaluated macroscopically and microscopically. Plasma nitric oxide (NO) concentration was determined by the Griess method and cytokine mRNA expression in the spleen was detected by the real-time reverse transcription-polymerase chain reaction (RT-PCR).
ISSN:1673-1581
DOI:10.1631/jzus.B1000078