Proteome Half-Life Dynamics in Living Human Cells

Cells remove proteins by two processes: degradation and dilution due to cell growth. The balance between these basic processes is poorly understood. We addressed this by developing an accurate and noninvasive method for measuring protein half-lives, called "bleach-chase," that is applicabl...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2011-02, Vol.331 (6018), p.764-768
Main Authors: Eden, Eran, Geva-Zatorsky, Naama, Issaeva, Irina, Cohen, Ariel, Dekel, Erez, Danon, Tamar, Cohen, Lydia, Mayo, Avi, Alon, Uri
Format: Article
Language:English
Subjects:
Anaphase-Promoting Complex-Cyclosome
Antineoplastic Agents - pharmacology
Antineoplastics
Biological and medical sciences
Bleaching
Camptothecin - pharmacology
Cancer
Cell cycle
Cell Cycle Proteins - metabolism
Cell Death
Cell division
Cell Division - drug effects
Cell growth
Cell Line, Tumor
Cell physiology
Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes
Cells
Cytoplasm - metabolism
Degradation
Diverse techniques
Dynamics
Fluorescence
Fundamental and applied biological sciences. Psychology
Half lives
Half-Life
Human
Humans
Killing
Light
Luminescent Proteins
Microscopy, Fluorescence
Molecular and cellular biology
Plugs
Proteins
Proteins - metabolism
Proteome - metabolism
Proteomes
Radioactive decay
rev genes
Stress, Physiological
Ubiquitin-Protein Ligase Complexes - metabolism
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Summary:Cells remove proteins by two processes: degradation and dilution due to cell growth. The balance between these basic processes is poorly understood. We addressed this by developing an accurate and noninvasive method for measuring protein half-lives, called "bleach-chase," that is applicable to fluorescently tagged proteins. Assaying 100 proteins in living human cancer cells showed half-lives that ranged between 45 minutes and 22.5 hours. A variety of stresses that stop cell division showed the same general effect: Long-lived proteins became longer-lived, whereas short-lived proteins remained largely unaffected. This effect is due to the relative strengths of degradation and dilution and suggests a mechanism for differential killing of rapidly growing cells by growth-arresting drugs. This approach opens a way to understand proteome half-life dynamics in living cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1199784