Adsorption behavior of 17α-ethynylestradiol onto soils followed by fluorescence spectral deconvolution

• Published in: Chemosphere (Oxford) 2011-08, Vol.84 (8), p.1072-1078
• Main Authors: Lima, Diana L.D., Calisto, Vânia, Esteves, Valdemar I.
• Format: Article
• Language: English
• Subjects: 17α-ethynylestradiol; Adsorption; Applied sciences; Deconvolution; Emission; emissions; energy; Environmental Restoration and Remediation; equations; Ethinyl Estradiol - chemistry; Exact sciences and technology; Fluorescence; Fluorescent Dyes - chemistry; Mathematical models; Models, Molecular; Molecular fluorescence; Molecular modelling; molecular models; plateaus; Pollution; soil; Soil - chemistry; soil sampling; Soils; Spectral emissivity; Spectral lines; Spectrophotometry, Ultraviolet
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2011.04.060

► FSD can be an alternative to UVSD for molecules with low UV–Vis absorbance. ► EE2 sorption isotherm presented an S shaped curve with good fit using Hill equation. ► Co-operative adsorption might occur due to association between EE2 molecules. ► Molecular modelling studies clearly support the co-operative adsorption model. In this study, a simple and rapid procedure for monitoring adsorption of 17α-ethynylestradiol (EE2) onto soil samples was developed. The used method is based on a multiwavelength fluorescence spectral deconvolution (FSD) where the emission fluorescence spectrum of a sample is considered as a linear combination of emission spectra, named reference spectra. The combination of the reference spectra allows the restitution of the shape of the emission spectrum of any unknown sample. This approach was applied to follow EE2 adsorption onto four soil samples and is an easy and low cost alternative. Adsorption experimental data showed a good fit with the Hill equation, mathematically equivalent to the Langmuir–Freundlich model assuming that the adsorption is a cooperative process influenced by adsorbate–adsorbate interactions. Molecular modelling studies clearly support the “co-operative adsorption” model, showing that after the adsorption of the first layer of EE2 molecules onto the soil, at least one more layer of EE2 is adsorbed, due to interactions established with the first adsorbed layer. Notwithstanding, packing a third row would imply interactions between two EE2 molecules that differ from the ones verified in the lowest energy structure, which also explains the plateau achieved in the adsorption curve.