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The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer
Destruction of cancer cells by cytotoxic T lymphocytes depends on immunogenic tumor peptides generated by proteasomes and presented by human leukocyte antigen (HLA) molecules. Functional differences arising from alleles of immunoproteasome subunits have not been recognized so far. We analyzed the ge...
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Published in: | Cancer research (Chicago, Ill.) Ill.), 2011-12, Vol.71 (23), p.7145-7154 |
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description | Destruction of cancer cells by cytotoxic T lymphocytes depends on immunogenic tumor peptides generated by proteasomes and presented by human leukocyte antigen (HLA) molecules. Functional differences arising from alleles of immunoproteasome subunits have not been recognized so far. We analyzed the genetic polymorphism of the immunoproteasome subunits LMP2 and LMP7 and of the transporters associated with antigen processing (TAP1 and TAP2) in two independently collected panels of colorectal carcinoma patients (N(1) = 112, N(2) = 62; controls, N = 165). High risk of colon cancer was associated with the LMP7-K/Q genotype (OR = 8.10, P = 1.10 × 10(-11)) and low risk with the LMP7-Q/Q genotype (OR = 0.10, P = 5.97 × 10(-13)). The basis for these distinct associations of LMP7 genotypes was functionally assessed by IFN-γ stimulation of colon carcinoma cell lines (N = 10), followed by analyses of mRNA expression of HLA class I, TAP1, TAP2, and LMP7, with real-time PCR. Whereas induction of HLA-B, TAP1, and TAP2 was comparable in all cell lines, transcript amounts of LMP7-Q increased 10-fold, but of LMP7-K only 3.8-fold. This correlated with a reduced transcript stability of LMP7-K (t(1/2) ≈ 7 minutes) compared with LMP7-Q (t(1/2) ≈ 33 minutes). In addition, LMP7-Q/Q colon carcinoma cells increased (the peptide based) HLA class I surface expression significantly after IFN-γ stimulation, whereas LMP7-Q/K and LMP7-K/K carcinoma cells showed minimal ( |
doi_str_mv | 10.1158/0008-5472.CAN-10-1883 |
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Functional differences arising from alleles of immunoproteasome subunits have not been recognized so far. We analyzed the genetic polymorphism of the immunoproteasome subunits LMP2 and LMP7 and of the transporters associated with antigen processing (TAP1 and TAP2) in two independently collected panels of colorectal carcinoma patients (N(1) = 112, N(2) = 62; controls, N = 165). High risk of colon cancer was associated with the LMP7-K/Q genotype (OR = 8.10, P = 1.10 × 10(-11)) and low risk with the LMP7-Q/Q genotype (OR = 0.10, P = 5.97 × 10(-13)). The basis for these distinct associations of LMP7 genotypes was functionally assessed by IFN-γ stimulation of colon carcinoma cell lines (N = 10), followed by analyses of mRNA expression of HLA class I, TAP1, TAP2, and LMP7, with real-time PCR. Whereas induction of HLA-B, TAP1, and TAP2 was comparable in all cell lines, transcript amounts of LMP7-Q increased 10-fold, but of LMP7-K only 3.8-fold. This correlated with a reduced transcript stability of LMP7-K (t(1/2) ≈ 7 minutes) compared with LMP7-Q (t(1/2) ≈ 33 minutes). In addition, LMP7-Q/Q colon carcinoma cells increased (the peptide based) HLA class I surface expression significantly after IFN-γ stimulation, whereas LMP7-Q/K and LMP7-K/K carcinoma cells showed minimal (<20%) changes. These results suggest that the presence of LMP7-K can reduce the formation of immunoproteasomes and thus peptide processing, followed by reduced peptide-HLA presentation, a crucial factor in the immune response against cancer.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/0008-5472.CAN-10-1883</identifier><identifier>PMID: 22037870</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adult ; Aged ; Alleles ; Antineoplastic agents ; ATP-Binding Cassette Sub-Family B Member 2 ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - immunology ; ATP-Binding Cassette, Sub-Family B, Member 3 ; Biological and medical sciences ; Caco-2 Cells ; Cell Line, Tumor ; Colonic Neoplasms - genetics ; Colonic Neoplasms - immunology ; Colonic Neoplasms - pathology ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - immunology ; Colorectal Neoplasms - metabolism ; Cysteine Endopeptidases - genetics ; Cysteine Endopeptidases - immunology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Regulation, Neoplastic - immunology ; Gene Frequency - immunology ; Genes, MHC Class I - genetics ; Genes, MHC Class I - immunology ; Genetic Predisposition to Disease ; Genotype ; HeLa Cells ; HLA-B Antigens - genetics ; HLA-B Antigens - immunology ; HT29 Cells ; Humans ; Immunogenetics - methods ; Interferon-gamma - genetics ; Interferon-gamma - immunology ; Interferon-gamma - metabolism ; Male ; Medical sciences ; Pharmacology. Drug treatments ; Polymorphism, Genetic ; Proteasome Endopeptidase Complex - genetics ; Proteasome Endopeptidase Complex - immunology ; RNA, Messenger - genetics ; RNA, Messenger - immunology ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 2011-12, Vol.71 (23), p.7145-7154</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-bde2baff1491760321ca824648a9a4301e0727e2dc19b4266252ec876610f6f33</citedby><cites>FETCH-LOGICAL-c418t-bde2baff1491760321ca824648a9a4301e0727e2dc19b4266252ec876610f6f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25250473$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22037870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FELLERHOFF, Barbara</creatorcontrib><creatorcontrib>SONGHAI GU</creatorcontrib><creatorcontrib>LAUMBACHER, Barbara</creatorcontrib><creatorcontrib>NERLICH, Andreas G</creatorcontrib><creatorcontrib>WEISS, Elisabeth H</creatorcontrib><creatorcontrib>GLAS, Jürgen</creatorcontrib><creatorcontrib>KOPP, Reinhard</creatorcontrib><creatorcontrib>JOHNSON, Judith P</creatorcontrib><creatorcontrib>WANK, Rudolf</creatorcontrib><title>The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Destruction of cancer cells by cytotoxic T lymphocytes depends on immunogenic tumor peptides generated by proteasomes and presented by human leukocyte antigen (HLA) molecules. Functional differences arising from alleles of immunoproteasome subunits have not been recognized so far. We analyzed the genetic polymorphism of the immunoproteasome subunits LMP2 and LMP7 and of the transporters associated with antigen processing (TAP1 and TAP2) in two independently collected panels of colorectal carcinoma patients (N(1) = 112, N(2) = 62; controls, N = 165). High risk of colon cancer was associated with the LMP7-K/Q genotype (OR = 8.10, P = 1.10 × 10(-11)) and low risk with the LMP7-Q/Q genotype (OR = 0.10, P = 5.97 × 10(-13)). The basis for these distinct associations of LMP7 genotypes was functionally assessed by IFN-γ stimulation of colon carcinoma cell lines (N = 10), followed by analyses of mRNA expression of HLA class I, TAP1, TAP2, and LMP7, with real-time PCR. Whereas induction of HLA-B, TAP1, and TAP2 was comparable in all cell lines, transcript amounts of LMP7-Q increased 10-fold, but of LMP7-K only 3.8-fold. This correlated with a reduced transcript stability of LMP7-K (t(1/2) ≈ 7 minutes) compared with LMP7-Q (t(1/2) ≈ 33 minutes). In addition, LMP7-Q/Q colon carcinoma cells increased (the peptide based) HLA class I surface expression significantly after IFN-γ stimulation, whereas LMP7-Q/K and LMP7-K/K carcinoma cells showed minimal (<20%) changes. These results suggest that the presence of LMP7-K can reduce the formation of immunoproteasomes and thus peptide processing, followed by reduced peptide-HLA presentation, a crucial factor in the immune response against cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Alleles</subject><subject>Antineoplastic agents</subject><subject>ATP-Binding Cassette Sub-Family B Member 2</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - immunology</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 3</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - immunology</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - immunology</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Cysteine Endopeptidases - genetics</subject><subject>Cysteine Endopeptidases - immunology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Regulation, Neoplastic - immunology</subject><subject>Gene Frequency - immunology</subject><subject>Genes, MHC Class I - genetics</subject><subject>Genes, MHC Class I - immunology</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotype</subject><subject>HeLa Cells</subject><subject>HLA-B Antigens - genetics</subject><subject>HLA-B Antigens - immunology</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Immunogenetics - methods</subject><subject>Interferon-gamma - genetics</subject><subject>Interferon-gamma - immunology</subject><subject>Interferon-gamma - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymorphism, Genetic</subject><subject>Proteasome Endopeptidase Complex - genetics</subject><subject>Proteasome Endopeptidase Complex - immunology</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - immunology</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxa0KRJeWj1DkC4JLiv_bOa6i0lZdaFWWc-Q4E9bgJIudoPbb19tuy43TaEa_eaN5D6ETSk4pleYzIcQUUmh2Wi2_FZQU1Bh-gBZUclNoIeQrtHhhDtHblH7lVlIi36BDxgjXRpMF-rveAF59vdHFFV6GAAHw2OEpDy_7fh7GbRwnsGnsAZ_dbXzjp4RvoZ0dtHgd7ZBc9NsJf59s44Of7rEdWnwTofUukxf-5wbf-vR7J1qNYRxwZQcH8Ri97mxI8G5fj9CPL2fr6qJYXZ9fVstV4QQ1U9G0wBrbdVSUVCvCGXXWMKGEsaUVnFAgmmlgraNlI5hSTDJwRitFSac6zo_Qxyfd_MefGdJU9z45CMEOMM6pLokhJKubTH76L0m15EKpUsqMyifUxTGlCF29jb638b6mpN6FU--Mr3fG1zmcx2kOJ--935-Ymx7al63nNDLwYQ_Y5Gzosr_Op3-cZJIIzfkDpcGV3Q</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>FELLERHOFF, Barbara</creator><creator>SONGHAI GU</creator><creator>LAUMBACHER, Barbara</creator><creator>NERLICH, Andreas G</creator><creator>WEISS, Elisabeth H</creator><creator>GLAS, Jürgen</creator><creator>KOPP, Reinhard</creator><creator>JOHNSON, Judith P</creator><creator>WANK, Rudolf</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer</title><author>FELLERHOFF, Barbara ; SONGHAI GU ; LAUMBACHER, Barbara ; NERLICH, Andreas G ; WEISS, Elisabeth H ; GLAS, Jürgen ; KOPP, Reinhard ; JOHNSON, Judith P ; WANK, Rudolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-bde2baff1491760321ca824648a9a4301e0727e2dc19b4266252ec876610f6f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Alleles</topic><topic>Antineoplastic agents</topic><topic>ATP-Binding Cassette Sub-Family B Member 2</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - immunology</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 3</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - immunology</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - immunology</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Cysteine Endopeptidases - genetics</topic><topic>Cysteine Endopeptidases - immunology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Regulation, Neoplastic - immunology</topic><topic>Gene Frequency - immunology</topic><topic>Genes, MHC Class I - genetics</topic><topic>Genes, MHC Class I - immunology</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotype</topic><topic>HeLa Cells</topic><topic>HLA-B Antigens - genetics</topic><topic>HLA-B Antigens - immunology</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Immunogenetics - methods</topic><topic>Interferon-gamma - genetics</topic><topic>Interferon-gamma - immunology</topic><topic>Interferon-gamma - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymorphism, Genetic</topic><topic>Proteasome Endopeptidase Complex - genetics</topic><topic>Proteasome Endopeptidase Complex - immunology</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - immunology</topic><topic>Stomach. Duodenum. Small intestine. 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Anus</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FELLERHOFF, Barbara</creatorcontrib><creatorcontrib>SONGHAI GU</creatorcontrib><creatorcontrib>LAUMBACHER, Barbara</creatorcontrib><creatorcontrib>NERLICH, Andreas G</creatorcontrib><creatorcontrib>WEISS, Elisabeth H</creatorcontrib><creatorcontrib>GLAS, Jürgen</creatorcontrib><creatorcontrib>KOPP, Reinhard</creatorcontrib><creatorcontrib>JOHNSON, Judith P</creatorcontrib><creatorcontrib>WANK, Rudolf</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FELLERHOFF, Barbara</au><au>SONGHAI GU</au><au>LAUMBACHER, Barbara</au><au>NERLICH, Andreas G</au><au>WEISS, Elisabeth H</au><au>GLAS, Jürgen</au><au>KOPP, Reinhard</au><au>JOHNSON, Judith P</au><au>WANK, Rudolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>71</volume><issue>23</issue><spage>7145</spage><epage>7154</epage><pages>7145-7154</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Destruction of cancer cells by cytotoxic T lymphocytes depends on immunogenic tumor peptides generated by proteasomes and presented by human leukocyte antigen (HLA) molecules. Functional differences arising from alleles of immunoproteasome subunits have not been recognized so far. We analyzed the genetic polymorphism of the immunoproteasome subunits LMP2 and LMP7 and of the transporters associated with antigen processing (TAP1 and TAP2) in two independently collected panels of colorectal carcinoma patients (N(1) = 112, N(2) = 62; controls, N = 165). High risk of colon cancer was associated with the LMP7-K/Q genotype (OR = 8.10, P = 1.10 × 10(-11)) and low risk with the LMP7-Q/Q genotype (OR = 0.10, P = 5.97 × 10(-13)). The basis for these distinct associations of LMP7 genotypes was functionally assessed by IFN-γ stimulation of colon carcinoma cell lines (N = 10), followed by analyses of mRNA expression of HLA class I, TAP1, TAP2, and LMP7, with real-time PCR. Whereas induction of HLA-B, TAP1, and TAP2 was comparable in all cell lines, transcript amounts of LMP7-Q increased 10-fold, but of LMP7-K only 3.8-fold. This correlated with a reduced transcript stability of LMP7-K (t(1/2) ≈ 7 minutes) compared with LMP7-Q (t(1/2) ≈ 33 minutes). In addition, LMP7-Q/Q colon carcinoma cells increased (the peptide based) HLA class I surface expression significantly after IFN-γ stimulation, whereas LMP7-Q/K and LMP7-K/K carcinoma cells showed minimal (<20%) changes. These results suggest that the presence of LMP7-K can reduce the formation of immunoproteasomes and thus peptide processing, followed by reduced peptide-HLA presentation, a crucial factor in the immune response against cancer.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>22037870</pmid><doi>10.1158/0008-5472.CAN-10-1883</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Aged Alleles Antineoplastic agents ATP-Binding Cassette Sub-Family B Member 2 ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - immunology ATP-Binding Cassette, Sub-Family B, Member 3 Biological and medical sciences Caco-2 Cells Cell Line, Tumor Colonic Neoplasms - genetics Colonic Neoplasms - immunology Colonic Neoplasms - pathology Colorectal Neoplasms - genetics Colorectal Neoplasms - immunology Colorectal Neoplasms - metabolism Cysteine Endopeptidases - genetics Cysteine Endopeptidases - immunology Female Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Regulation, Neoplastic - immunology Gene Frequency - immunology Genes, MHC Class I - genetics Genes, MHC Class I - immunology Genetic Predisposition to Disease Genotype HeLa Cells HLA-B Antigens - genetics HLA-B Antigens - immunology HT29 Cells Humans Immunogenetics - methods Interferon-gamma - genetics Interferon-gamma - immunology Interferon-gamma - metabolism Male Medical sciences Pharmacology. Drug treatments Polymorphism, Genetic Proteasome Endopeptidase Complex - genetics Proteasome Endopeptidase Complex - immunology RNA, Messenger - genetics RNA, Messenger - immunology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
title | The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer |
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