Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats

• Published in: Cell biochemistry and function 2011-12, Vol.29 (8), p.630-635
• Main Authors: Jaques, Jeandre Augusto dos Santos, Ruchel, Jader Betsch, Schlemmer, Karine Bizzi, Pimentel, Victor Camera, Bagatini, Margarete, Souza, Viviane do Carmo Gonçalves, Moretto, Maria Beatriz, Morsch, Vera Maria, Schetinger, Maria Rosa Chitolina, Leal, Daniela Bitencourt Rosa
• Format: Article
• Language: English
• Subjects: 5'-Nucleotidase - genetics; 5'-Nucleotidase - metabolism; Adenine Nucleotides - metabolism; Adenosine Deaminase - genetics; Adenosine Deaminase - metabolism; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Apyrase - genetics; Apyrase - metabolism; Blood Platelets - enzymology; Blood Platelets - metabolism; cigarette smoke; Curcuma; curcumin; Curcumin - pharmacology; Enzyme Activation - drug effects; Humans; Male; Nicotiana - adverse effects; Plant Extracts - pharmacology; Platelet Aggregation - drug effects; platelets; purinergic signalling; Rats; Rats, Wistar; Smoking - adverse effects
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1798

The aim of the present study was to investigate the effect of curcumin (Cur) on the activity of ectonucleoside triphosphate diphosphohydrolase (CD39), 5'‐nucleotidase (CD73) and adenosine deaminase in platelets of cigarette smoke‐exposed rats. For that purpose, we subjected male Wistar rats to a treatment with Cur and cigarette smoke, once a day, 5 days each week, for 30 days. The rats were treated by gavage with Cur or corn oil and then exposed to cigarette smoke. The experimental procedures were divided into two sets of experiments. In the first, the animals were divided into four groups: vehicle (corn oil) or Cur 12·5, 25 or 50 mg·kg‐1. In the second, the animals were divided into five groups: vehicle (corn oil), smoke, or smoke and Cur 12·5, 25 or 50 mg·kg‐1. The results showed that treatment with Cur significantly prevented the increased adenosine triphosphate (ATP) (121%) and adenosine monophosphate (AMP) (159%) and the decreased adenosine diphosphate (ADP) (51%) hydrolysis observed in the cigarette smoke‐exposed rats Our results suggest that those purinergic enzyme alterations observed in the cigarette smoke‐exposed rats could be related to an excessive platelet aggregation and point toward the potential of Cur to modulate purinergic signalling and, consequently, regulate the thrombus formation. Copyright © 2011 John Wiley & Sons, Ltd.